Cell imaging techniques :methods and protocols /edited by Douglas J. Taatjes, Brooke T. Mossman. --Humana Press, 2006. --(58.17/M592/v.319)

Contents

Contents:

1. Molecular Beacons: Fluorescent Probes for Detection of Endogenous

    mRNAs in Living Cells  1

2. Second-Harmonic Imaging of Collagen  15

3. Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal

     Fluorescent Microscopy  37

4. Multifluorescence Labeling Techniques and Confocal Laser

    Scanning Microscopy on Lung Tissue  67

5. Evaluation of Confocal Microscopy System Performance  77

6. Quantitative Analysis of Atherosclerotic Lesion Composition in Mice  137

7. Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other

    Human Diseases  137

8. Laser Scanning Cytometry: Principles and Applications  165

9. Near-Clinical Applications of Laser Scanning Cytometry  193

10. Laser Capture Microdissection  213

11. Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar

      Epithelial Cells Using laser Capture Microdissection and Quantitative

      Reverse Transcriptase-Polymerase Chain Reaction  231

12.New Approaches to Fluorescence In Situ Hybridization  237

13. Microarray Image Scanning  261

14. Near-Field Scanning Optical Microscopy in Cell Biology and

      Cytogenetics  275

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