Methods in cell biology .v. 77 /edited by H. William Detrich, Monte Westerfield, Leonard In Zon. --Elsevier, 2004. -- (58.1574/M592/v.77)

Contents

Contents:

1.       Retroviral-Mediated Insertional Mutagenesis in Zebrafish  3

2.       Genetic Screens for Maternal-Effect Mutations  22

3.       Behavioral Screening Assays in Zebrafish  53

4.       Target-Selected Gene Inactivation in Zebrafish  70

5.       A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish  92

6.       Production of Zebrafish Germline Chimeras by Using Cultured Embryonic

Stem(ES) Cells  113

7.       Nonconventional Antisense in Zebrafish for Functional Genomics Applications  121

8.       Downregulation of Gene Expression with Negatively Charged Peptide Nucleic Acids (PNAs) in Zebrafish Embryos  138

9.       Photo-Mediated Gene Activation by Using Caged mRNA  in Zebrafish Embryos  160

10.   Current Status of Medaka Genetics and Genomics  174

11.   Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element  201

12.   The Zebrafish Genome Project: Sequence Analysis and Annotation  225

13.   Molecular Cytogenetic Methodologies and a Bacterial Artifical Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish  241

14.   Automated Analysis of Conserved Syntenies for the Zebrafish Genome  260

15.   Update of the Expressed Sequence Tag (EST) and Radiation Hybrid Panel Projects  274

16.   Bacterial Artificial Chromosome (BAC) Clones and the Current Clone Map of the Zebrafish Genome  296

17.   The Zon Laboratory Guide to Positional Cloning in Zebrafish  306

18.   Lessons from Transgenic Zebrafish Expressing the Green Fluorescent Protein (GFP) in the Myeloid Lineage  334

19.   Sleeping Beauty Transponson for Efficient Gene Delivery  349

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