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Phosphodiesterase methods and protocols /edited by Claire Lugnier. -- Humana Press, 2005. -- (58.17/M592/V.307) |
Contents
Contents:
1.Study of Cyclic Adenosine Monophosphate Microdomains in Cells 1
2.High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains 15
3.Cygnets: In Vivo Imaging of Intracellular cGMP 27.
4.High-Throughput Screening of Phosphodiesterase Activity in Living Cells 45
5.Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts 63
6.Localization of the Cyclic Guanosine 3',5'-Monophosphate-Hydrolyzing Phosphodiesterase Type
9 in Rat Brain by Nonradioactive in Situ Hybridization 75
7.Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells 85
8.Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B 93
9.Identification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase
3B Gene 109
10.Purification of PDE6 Isozymes From Mammalian Retina 125
11.Cyclic Guanosine 5'-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase 141
12.Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies 141
13.Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation 167
14.Crystallization of Cyclic Nucleotide Phosphodiesterases 181
15.Generation of PDE4 Knockout Mice by Gene Targeting 191
16.Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase 211
17.Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases 225
18.Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 239
19.Analysis of Dimerization Determinants of the PDE6 239
20.Interaction Between Catalytic and Inhibitory Subunits of PDE6
21.Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and its Activator G Protein, Transducin 289
