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Cytokine Protocols / edited by Marc De Ley. -- (Methods in Molecular Biology ; v. 249). -- Humana Press, 2004

Contents

索书号: 58.17/M592/v.249

Contents:

1.Large-Scale Generation of Plasmids that Express Types I Interferon  1

2.Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay  7

3.Modulation of the Interferon- γ Signal by Transfection of Cells with an Antisense-RNA Expressing Vector  21

4.Competitive RT-PCR to Quantify Small Amounts of mRNA  31

5.In Situ Hybridization for Cytokines in Human Tissue Biopsies  41

6.Purification, Identification, and Synthesis of Chemokines  47

7.Receptor Isolation and Characterization: From Protein to Gene  65

8.Crystallization of Cytokine-Receptor Complexes  81

9.Biosensor Analysis of Receptor-Ligand Interactions  93

10.analysis of SH2 Ligands and Indentification of Sites of Interaction  111

11.Assays for Antiviral Activity  121

12.Assays for Cytotoxicity  135

13.Assays for Chemotaxis  153    

14.In Vitro and In Vivo Assays for High-Molecular-Weight Prgylated Muteins of Granulocyte Colony-Stimulating Factor  167

15.Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-α  -Induced Apoptosis  177

16.Immunohistochemical Detection of Cytokines in Human Tissue Sections  193

17.Detecting of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level  201

18.Detection of Cytokine Signal Transduction "Cross-Talk" in Leukocyte Activation  219

19. Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production  229