Cytokine Protocols / edited by Marc De Ley. -- (Methods in Molecular Biology ; v. 249). -- Humana Press, 2004 |
Contents
索书号: 58.17/M592/v.249 Contents: 1.Large-Scale Generation of Plasmids that Express Types I Interferon 1 2.Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay 7 3.Modulation of the Interferon- γ Signal by Transfection of Cells with an Antisense-RNA Expressing Vector 21 4.Competitive RT-PCR to Quantify Small Amounts of mRNA 31 5.In Situ Hybridization for Cytokines in Human Tissue Biopsies 41 6.Purification, Identification, and Synthesis of Chemokines 47 7.Receptor Isolation and Characterization: From Protein to Gene 65 8.Crystallization of Cytokine-Receptor Complexes 81 9.Biosensor Analysis of Receptor-Ligand Interactions 93 10.analysis of SH2 Ligands and Indentification of Sites of Interaction 111 11.Assays for Antiviral Activity 121 12.Assays for Cytotoxicity 135 13.Assays for Chemotaxis 153 14.In Vitro and In Vivo Assays for High-Molecular-Weight Prgylated Muteins of Granulocyte Colony-Stimulating Factor 167 15.Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-α -Induced Apoptosis 177 16.Immunohistochemical Detection of Cytokines in Human Tissue Sections 193 17.Detecting of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level 201 18.Detection of Cytokine Signal Transduction "Cross-Talk" in Leukocyte Activation 219 19. Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production 229 |