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Developmental Hematopoiesis : methods and protocols /cedited by Margaret H. Baron. --Humana Press, 2005. (59.524/D444)

Contents

Contents:

1. Gene-Targeting and Transgenic Strategies for the Analysis of hematopoietic Development in the Mouse  3

2. Inducible Transgene Expression in Mouse ES Cells  23

3. Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic

     Stem Cells  47

4. Flow Cytometric Analysis of Hematopoietic Development  65

5. Reconstitution of Hematopoiesis following Intrauterine Transplantation of Stem Cells  81

6. Reconstitution of Hematopoiesis Following Transplantation into Neonatal Mice  95

7. Hemocyte Development During Drosophila Embryogenesis  109

8. Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos  123

9. Fate Mapping Hematopoietic Lineages in the Xenopus Embryo  137

10. Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus Laevis (J Strain)  149

11. Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo  159

12. Analysis of Hematopoietic Development in the Zebrafish  171

13. Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live

       Zebrafish Embryos  199 

14.  In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo  215

15. Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular        

      Deve lopment  231

16. Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo  257

17.Hematopoietic Stem Cell Development During Mouse Embryogenesis  273

18.Analysis of Hematopoietic Progenitors in the Mouse Embryo  289

19.In Vivo and in Vitro Assays of thymic Organogenesis  303

20. Retrovial Transduction in Fetal Thymic Organ Culture  311

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