Receptor binding techniques / edited by Anthony P. Davenport . -- 2nd ed. -- (Methods in molecular biology ; v. 306). -- Humana Press, 2005. (58.17/M592/v.306)

Contets:

1. Receptor Databases and Computation Websites for Ligand Binding  1

2. Identification of Orphan G Protein-Coupled Receptro Ligands Using FLIPR Assays  17

3. Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan Real-Time PCR: G2A and GPR4 Lysophospholipid Receptor Expression in Leukocytes and in a Rat Myocardial Infarction-Heart Failure Model  27

4. mRNA: Detection by In Situ and Northern Hybridization  51

5. Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative analysis of Established and Emerging Orphan Receptor systems  993

6. Measurement of Radiologand binding  by Scintillation Proximity Assay  121

7. Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels   139

8. Immunocytochemical Localization of Receptors Using Light and confocal Microscopy With application to the Phenotypic Characterization of Knock-Out mice  155

9. Live Cell Imaging of G. Protein-Coupled Receptors  173

10. Imaging and Charaterization of Radioligands for Positron Emission Tomography Using Quantitative Phosphor Imaging Autoradiography  203

11. Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography