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Handbook of ELISPOT : methods and protocols / edited by Alexander E. Kalyuzhny. -- (Methods in molecular biology ; v. 302). -- Humna Press, 2005. (58.17/M592/v.302) |
Contents
Contents: 1. The ELISPOT Assay : a Personal Retrospective 3 2. Chemistry and biology of the ELISPOT Assay 15 3. Membranes and Membrane Plates Used in ELISPOT 33 4. Standardization and Validation Issues of the ELISPOT Assay 51 5. A Cell-Detachment solution Can Reduce Background Staining in the ELISPOT Assay 87 6. Isolation of Subsets of Immune Cells 95 7. Image analysis and Data management of ELISPOT Assay Results 117 8. High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers 133 9. Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques 153 10. Feline Cytokine ELISPOT: Issues in Assay Development 167 11. Measuring T-Cell Function in animal Models of Tuberculosis by ELISPOT 179 12. Interferon-Y Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+T-Cell 191 13. Detection of Measles Virue-specific Interferon-Y-Secreting T-Cells by ELISPOT 207 14. Epitope Mapping in Multiple sclerosis Using the ELISPOT Assay 219 15. Use of Interferon-Y ELISPOT in Monitoring Immune esponses in Humans 237 16. ELISPOT Determination of Interferon-Y T-Cell Frequencies in patients With Autoimmune Sensorineural Hearing Loss 253 17. Dual-Color ELISPOT Assay for Analyzing Cytokine Balance 263 18. Simultaneous Detection of Multiple Cytokines in ELISPOT Assays 273 19. Fluorospot Assay: Methodological analysis 289 20. A Gel-Based Dual antibody Capture and Detection Method for Assaying of Extracellular cytokine Secretion: EliCell 297 |
