Ubiquitin-proteasome protocols / edited by Cam Patterson, Douglas M Cyr. -- (Methods in molecular biology ; v. 301). -- Humana Press, 2005. (58.17/M592/v.301)

Contents:

1. Small-Molecule Inhibitors of Proteasome Activity  3

2. Purification of E1 and E1-Like Enzymes  23

3. Assays for RING Family Ubiquitin Ligases  37

4. Ubiquitin Chain synthesis  47

5. Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated proteins From Budding yeast  57

6. Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome  71

7. Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation  83

8. Assays of Proteasome-Dependent Cleavage Products  97

9. Identification of Components of Protein Complexes  117

10. Mass Spectrometric Determination of Protein Ubiquitination  153

11. Reconstitution of endoplasmic Reticulum-Associated Degradation Using Yeast membranes and Cytosol  175

12. Reticulocyte Lysate as a Model System to Study Endoplasmic  185

13. Deubiquitinating Enzyme purification, Assay Inhibitors, and Characterization  207

14. Measuring Ubiquitin Conjugation in Cells  223

15. Assays for Proteasome Assembly and Maturation  243

16. N-Terminal Ubiquitination  255

17. Quantitating Defective Ribosome Products  271

18. endoplasmic Reticulum-Associated Protein Qualith Control and Degradation: Screen for ERAD Mutants After Ethylmethane sulfonate Mutagenesis  283

19. endopladmic Reticulum-Associated Protein quality Control and Degradation: Genome Wide Svreen for ERAD Components  289

20. Cystic fibrosis Transmenbrane conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein quality control in Mammalian Cells  293

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