Amyloid proteins : methods and protocols / edited by Einar M. Sigurdsson. -- (Methods in molecular biology ; v. 299). -- Humana Press, 2005. -- (58.17/M592/v.299)

Contents:

1. Preparation of Aggregate-Free, Low Molecular Weight Amyloid- B for Assembly and Toxicity Assays  3

2. Determination fo Peptide Oligomerization State using Rapid Photochemical Crosslinking  11

3. In Virtro Preparation of prefibrillar Intermediates of Amyloid-B ans a-Synuclein  19

4. Purification fo Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical filaments in Vitro  35

5. Cyclic amplification of Protein Misfolding and Aggregation  53

6. X-Ray Diffaction Studies of Amyloid Structure  67

7. Molecular Electron Microscopy approaches to Elucidating the Mechanisms of Protein fibrillogenesis  81

8. Time-Lapse Atomic force Microscopy in the characterization of amyloid-Like Fibril Assembly and Oligomeric Intermediates  103

9. Fourier Transfom Infrared and Circular Dichroism spectroscopies for Amyloid Studies  129

10. quasielastic Light Scattering for Protein Assembly Studies  153

11. Intrinsic Fluorescent Detection of Tau conformation and Aggredation  175

12. Quantitative Measurement of Fibrillogenesis by mass Spectrometry  185

13. Isolation and Culturing of Human Vascular smooth Muscle Cells  197

14. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid angiopathy: isolation of Smooth Muscle and Endothelial Cells Media of Transfected Cells  221

15. Purification of Human Wild-type or Variant Cystatin C From conditioned Media of Transfected Cells  221

16. Prion Propagation in Cell culture  227

17. preparation and Propagation of Amyloid-Enhancing Factor  237

18. Purification of Amyloid Protein AA Subspecies From amyloid-Rich Human Tissues  243

19. Purification of Transthyretin and Transthyretin Fragments From amyloid-Rich Human Tissues  255

20. Extraction and Chemical characterization of Tissue-Deposited Proteins From Minute diagnostic Biopsy Specimens  261

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