Technology platforms for 3D cell culture : a user's guide / edited by Stefan Przyborski. -- Chichester, West Sussex : John Wiley & Sons, Inc., c2017. – (58.221 /T255)

Contents

List of contributors, vii

Preface, xi

List of abbreviations, xiv

1 An introduction to the third dimension for routine cell culture, 1

Part I: Aggregate-based technologies

2 Gravity-enforced microtissue engineering, 23

3 Physiologically relevant spheroid models for three-dimensional cell culture, 50

4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture, 74

5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues - the 3D Petri Dish, 97

6 0rganotypic microtissues on an air-liquid interface, 123

Part II: Hydrogels

7 Materials and assay systems used for three-dimensional cell culture, 145

8 HyStem, a customisable hyaluronan-based hydrogel matrix for 3D cell culture, 173

9 3-D Life biomimetic hydrogels: A modular system for cell environment design, 197

Part III: Scaffolds

10 Alvetex, a highly porous polystyrene scaffold for routine three-dimensional cell culture, 225

11 CelluSpongeTM and Go Matrix as innovative three-dimensional cell culture platforms, 250

12 Mimetix electrospun scaffold: An easy-to-use tool for 3D cell culture in drug discovery and regenerative medicine, 284

Part IV: 3D bioreactor technologies

13 Quasi Vivo bioreactor technology, 305

14 Three-dimensional cell-based assays in hollow fibre bioreactors, 327

15 Three-dimensional engineered tissues for high-throughput compound screening: Mechanical properties of skin and ageing, 351

16 Three-dimensional cell culture in the Rotary Cell Culture SystenfTM, 370

Glossary, 386

Index, 393