The neuronal cytoskeleton, motor proteins, and organelle trafficking in the axon / edited by K. Kevin Pfister. -- Cambridge, Massachusetts : Academic Press, c2016. – (58.1574/M592/v.131)

Contents

Contributors

Preface  xxi

CHAPTER 1  Axonal Transport: The Orderly Motion of Axonal Structures  1

CHAPTER 2 Live-cell imaging of Neurofilament Transport in Cultured Neurons  21

CHAPTER 3  Axonal Actin in Action: Imaging Actin Dynamics in Neurons  91

CHAPTER 4 Live Imaging of Microtubule Dynamics in Organotypic Hippocampal Slice Cultures  lO7

CHAPTER 5 Studying Neuronal Microtubule Organization and Microtubule-associated Proteins Using Single Molecule Localization Microscopy  127

CHAPTER 6 The Use of Proteomic Analysis to Study Trafficking Defects in Axons  151

CHAPTER 7 Using siRNA to Study Microtubule-related Proteins in Cultured Neurons  163

CHAPTER 8 Optimization of Cerebellar Purkinje Neuron Cultures and Development of a Plasmid-based Method for Purkinje Neuron-specific, miRNA-mediated Protein Knockdown  177

CHAPTER 9 Biochemical Analysis of Axon-specific Phosphorylation Events Using Isolated Squid Axoplasms  199

CHAPTER 10 Analyzing Kinesin Motor Domain Translocation in Cultured Hippocampal Neurons  217

CHAPTER 11 Imaging and Quantitative Analysis of Cytokinesis in Developing Brains of Kinesin-6 Mutant Mice  233

CHAPTER 12 Live Cell Imaging of Cytoplasmic Dynein Movement in Transfeeted Embryonic Rat Neurons  253

CHAPTER 13 Live-cell Imaging of Retrograde Transport Initiation in Primary Neurons  269

CHAPTER 14 Methods to Identify and Analyze Gene Products Involved in Neuronal Intracellular Transport Using Drosophila

CHAPTER 15 In Vivo Analysis of Axonal Transport in Zebrafish 311

CHAPTER 16 Fast Axonal Transport in Isolated Axoplasm From the Squid Giant Axon  331

CHAPTER 17 Cellular and Subcellular Imaging of Motor Protein-based Behavior in Embryonic Rat Brain  349

CHAPTER 18 Tracking Quantum-Dot Labeled Neurotropic Factors Transport Along Primary Neuronal Axons in Compartmental Microfluidic Chambers  365

CHAPTER 19 Studying Endosomes in Cultured Neurons by Live-cell Imaging  389

CHAPTER 20 Comparative Analysis of Axonal Transport Markers in Primary Mammalian Neurons  409

CHAPTER 21 Imaging Organelle Transport in Primary Hippocampal Neurons Treated with Amyloid-13 Oligomers  425

CHAPTER 22 Imaging of Motor-Dependent Transport in Neuronal and Nonneuronal Cells at High Spatial and Temporal Resolution  453

CHAPTER 23 Isolation and Analyses of Axonal Ribonucleoprotein Complexes  467

Volumes in Series  487

Index  501