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Cell membrane nanodomains : from biochemistry to nanoscopy / editors, Alessandra Cambi and Diane S. Lidke. -- Boca Raton : Taylor & Francis, 2015. – (58.1551/C393c)

Contents

Preface

Acknowledgments

Editors

Contributors

SECTION I

Protein and Lipid Nanodomains

Chapter 1  Giant Unilamellar Vesicles (GUVs) as a Laboratory to Study Mesoscopic Lipid Domains in Membranes  3

Chapter 2  An Active Basis for the Nanoscopic Organization of Membrane Components in Living Cell Membranes

Chapter 3  Functional Role of Membrane Lipids in EGF Receptor Dynamics and Regulation

Chapter 4  Tetraspanins as Master Organizers of the Plasma Membrane  59

Chapter 5  B Cell Receptor Signaling

Chapter 6  Imaging the Complexity, Plasticity, and Dynamics of Caveolae  113

Chapter 7  Membrane Microdomains Enriched in Ceramides: From Generation to Function

Chapter 8  Domains of Phosphoinositides in the Plasma Membrane  173

Chapter 9  Signaling Phagocytosis: Role of Specialized Lipid Domains

SECTION II  Advanced Ensemble Imaging Techniques

Chapter 10 Fluctuation Spectroscopy Methods for the Analysis of Membrane Processes

Chapter 11 Spatial Intensity Distribution Analysis (SpIDA): A Method to Probe Membrane Receptor Organization in Intact Cells  239

Chapter 12 Live-Cell TIRF Imaging of Molecular Assembly and Plasma Membrane Topography

SECTION III Expanding the Fluorescence Toolbox

Chapter 13 Laurdan Identifies Different Lipid Membranes in Eukaryotic Cells

Chapter 14 Development of Optical Highlighter Fluorescent Proteins and Their Applications in Super-Resolution Fluorescence Microscopy 305

Chapter 15 Targeting Dyes for Biology

SECTION IV  Nanoscopy

Chapter 16 Combined Topography, Recognition, and Fluorescence Measurements on Cells

Chapter 17 Super-Resolution Imaging with Single-Molecule Localization  391

Chapter 18 Visualization and Resolution in Localization Microscopy  409

Chapter 19 Molecular Plasma Membrane Dynamics Dissected by STED Nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS)  431

Chapter 20 Nanophotonic Approaches for Nanoscale Imaging and Single-Molecule Detection at Ultrahigh Concentrations  453

Index