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Introductory experiments on biomolecules and their interactions / Robert Delong, Angela Zhou. -- Boston, MA : Elsevier, c2015. – (58.178057/D361) |
Contents
Introduction
About the Authors
Master Materials List
Format of the Lab Notebook and Formal Lab Report
Lab Safety and Policies
1. An Introduction to Basic Math and Operations in
the Biomolecular Laboratory
Safety and Hazards
Introduction
Technical Review
Equipment Highlight
Experiment 1
Methods and Procedure
Results and Discussion
Problem Set
Additional Materials
2. Preparing Buffers at a Specific Molarity and pH
Safety and Hazards
Introduction
Technical Review
Equipment Highlight
Experiment 2
Results and Discussion
Problem Set
Additional Materials
3. Investigating the Physico-Chemical Properties of
Amino Acids and Their Analysis by Thin Layer Chromatography
Safety and Hazards
Introduction
Technical Review
Equipment Highlight
Experiment 3
Methods and Procedure
Results and Observations
Discussion
Problem Set
Acknowledgments
Additional Materials
4. Rapid Purification, Gel Electrophoresis, and
Enzyme Activity Assay of the Luciferase Enzyme from Fireflies
Safety and Hazards
Introduction
Technical Review and Equipment Highlights
Experiment 4
Results and Discussion
Problem Set
References
Additional Materials
5. Hexokinase and G6PDH Catalyzed Reactions of
Glucose Measurement
Safety and Hazards
Introduction
Technical Review
Equipment Highlight
Experiment 5
Results and Discussion
Problem Set
References
6. Polymerase Chain Reaction (PCR)
Safety and Hazards
Introduction
Technical Review
Experiment 6
Results and Discussion
Problem Set
Reference
7. Investigating Protein: Nucleic Acid Interactions
by Electrophoretic Mobility Shift Assay (EMSA)
Safety and Hazards
Introduction
Equipment Highlights
Experiment 7
Results and Discussion
Problem Set
References
8. Qualitative Analysis of the Degradation of RNA
via Ribonuclease A versus B
Safety and Hazards
Introduction
Experiment 8
Results and Discussion
Problem Set
References
9. Preparation of a Fluorescently Labeled Liposome
and Its Analysis by Fluorescence Microscopy
Safety and Hazards
Introduction
Experiment 9
Results and Discussion
Problem Set
References
10. Studying Cell-like Structures with Liposome,
DNA, and Protein
Safety and Hazards
Introduction
Experiment 10
Methods and Procedures
Results and Discussion
Additional Experiment
References
Index
