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How enzymes work : from structure to function / Haruo Suzuki. -- Singapore : Pan Stanford Publishing Pte. Ltd., c2015. – (58.17435/S968)

Contents

1.  Introduction

Contents

1.1  General Properties of Enzyme

1.2  Examples of Enzyme

2.  Overall Reaction Kinetics

2.1  Road to the Steady State Kinetics

2.2  Demonstration of the Enzyme-Substrate Complex

2.3  Meaning of Steady State

2.4  Kinetic Parameters

3.  Factors That Affect Enzyme Activity

3.1  Enzyme Concentration

3.2  Substrate Concentration

3.3  Inhibitor

4.  Effect of pH, Temperature, and High Pressure on Enzymatic Activity

4.1  EffectofpH

4.2  Thermodynamics in the Enzymatic Reaction

4.3  Temperature Dependence of the Enzymatic Reaction

4.4  Effect of Pressure

4.5  The Effect of Temperature and Pressure on a-Chymotrypsin-Catalyzed Reaction

5.  Measurement of Individual Rate Constants

5.1  Rapid-Mixing Techniques

5.2  Analysis of the First-Order Reaction

6.  Structure of Protein

6.1  Amino Acids

6.2  Polypeptide and Protein

6.3  Analysis of Primary Structure

6.4  Three-Dimensional Structure

6.5  Tertiary and Quaternary Structures

6.6  Structural Motif and Loop

7.  Active Site Structure                                117

7.1  Active Site and Active Center                     117

7.2  Cofactor, Coenzyme, Prosthetic Group              117

7.3  Search of Active Site

8.  Control of Enzyme Activity

8.1  Regulation byNon-Covalent Interaction

8.2  Regulation by Covalent Modification

9.  Preparation of Enzyme

9.1  Extraction of Enzyme

9.2  Purification of Enzyme

9.3  Purity Analysis of Enzyme

10. A Case Study: L-Phenylalanine Oxidase (Deaminating and Decarboxylating)

10.1 Introduction

10.2 Preparation of PAO

10.3 Catalytic Properties of PAO

10.4 Structural Properties of PAO

10.5 Substrate Specificity and Reaction Specificity of PAO

Appendix

Solutions

Index