Neural stem cell assays / edited by Navjot Kaur, Mohan C. Vemuri. -- Hoboken, New Jersey : Wiley Blackwell, c2015. – (59.5911/N494) |
Contents
Contributors
Preface
1 Neural
Differentiation of Pluripotent Stem Cells
1.1
Introduction
1.2 NSC Derivation
from Rosette Formation of Embryoid Bodies
1.3 Rosette
Free NSC in a Monolayer Culture
1.4 Pathways
Involved in Neural Tube and Neural Crest Lineages
1.5
Differentiation and Gene Expression in Human Brain Development
1.6
Conclusions
References
2 The
History and Design of Assays for the Identification and Characterization of
Neural Stem Cells
2.1
Introduction
2.2
Materials
2.3 Methods
2.4 Notes
Acknowledgments
References
3 Culture of
Human Neural Stem Cells
3.1
Introduction
3.2
Materials
3.3 Methods
3.4 Notes
3.5 Expected
Results
References
4 GMP4-Compatible
Production and Expansion of Human Neural Stem Cells
4.1
Introduction
4.2
Materials
4.3 Methods
4.4 Notes
Acknowledgments
References
5 Primary
Rat Neural Cultures
5.1
Introduction
5.2
Materials
5.3 Methods
5.4 Notes
References
6 Cryopreservation
of Human Neural Stem and Progenitor Cells
6.1
Introduction
6.2
Materials
6.3 Methods
6.4 Notes
References
7 Cryopreservation
and Recovery of Primary Rat Neural Cells
7.1 Introduction
7.2
Materials
7.3 Methods
7.4 Notes
References
8 Efficient
Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent
Stem Cells
8.1
Introduction
8.2
Materials
8.3 Methods
8.4 Notes
References
9 Directed
Differentiation of Human NSC/NPC into Dopaminergic Neurons
9.1
Introduction
9.2
Materials
9.3 Methods
9.4 Notes
References
10 In vitro
Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or
Midbrain Dopaminergic Neurons
10.1 Introduction
10.2 Materials
10.3 Methods
10.4 Notes
10.5 Typical
Results
References
11
Generation of Astrocytes from Human Piuripotent Stem Cells using a
Defined System
11.1
Introduction
11.2 Materials and Methods
11.3 Notes
References
12 Directed
Differentiation of Human PSC into Oligodendrocytes
12.1 Introduction
12.2 Materials
12.3 Methods
12.4 Notes
Acknowledgments
References
13 Directed
Differentiation towards Human Neural Retinal Cells
13.1
Introduction
13.2 Materials
13.3 Methods
References
14 Directed
Differentiation of Photoreceptors and Retinal Pigment Epithelium from Adult
Mouse Retinal Stem Cells
14.1
Introduction
14.2 Materials
14.3 Methods
14.4 Notes
Acknowledgments
References
15 Induction
of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells
15.1
Introduction
15.2 Materials
15.3 Method
15.4 Notes
References
16 Neural
Cell Viability Assays
16.1 Introduction
16.2 Materials
16.3 Methods
16.4 Notes
References
17 Genetic
Modification of Pluripotent Stem Cell Derived Human Neural Progenitor Cells
17.1
Introduction
17.2 Materials
17.3 Methods
17.4 Notes
Acknowledgments
References
18 Electrophysiology
of Neurons
18.1 Introduction
18.2 Materials
18.3 Methods
18.4 Notes
References
19 Induction
and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells in
Experimental Models (Rat) of Parkinson's disease
19.1
Introduction
19.2 Materials
19.3 Methods
19.4 Notes
Acknowledgments
References
20 Transplantation of Human Neural Stem Cells and
Progenitors in Animal Models of Disease
20.1
Introduction
20.2 General
Principles of Effective Transplantation
20.3 Different Methods of Transplantation
20.4 Materials and Method
20.5 Conclusions
20.6 Notes
Acknowledgments
References
21
Morphometric Assays for Neural Differentiation and Synaptie Development
of Human iPSC-Derived NSC
21.1 Introduction
21.2 Materials
21.3 Method
21.4 Notes
Acknowledgments
References
22 BacMam
Transduction of Neural Stem Cells
22.1
Introduction
22.2 Materials
22.3 Methods
22.4 Notes
Acknowledgments
References
23 The
Proximity Ligation Assay: A High Throughput Technique for Protein Analysis in
Neuroscience
23.1 Introduction
23.2 Materials
23.3 Methods
23.4 Notes
23.5 Troubleshooting
Acknowledgments
References
24 Quantification of Micro-RNA Expression in
Human Neural Stem Cells using TaqMan Assays
24.1
Introduction
24.2 Materials
24.3 Methods
24.4 Notes
References
25 Genetic
Reporter Cell Lines: Tools for Stem Cell Biology and Drug Discovery
25.1 Pluripotent Stem Cell Reporter Lines
25.2 Choice
of PSC Genetic Reporter Systems
25.3 Reporter Systems
25.4 Multiple Reporter Lines
25.5 Use of
Genetic Reporter Escs to Study Neural Differentiation
References
Index