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Neural stem cell assays / edited by Navjot Kaur, Mohan C. Vemuri. -- Hoboken, New Jersey : Wiley Blackwell, c2015. – (59.5911/N494)

Contents

Contributors

Preface

1  Neural Differentiation of Pluripotent Stem Cells

1.1  Introduction

1.2  NSC Derivation from Rosette Formation of Embryoid Bodies

1.3  Rosette Free NSC in a Monolayer Culture

1.4  Pathways Involved in Neural Tube and Neural Crest Lineages

1.5  Differentiation and Gene Expression in Human Brain Development

1.6  Conclusions

References

2  The History and Design of Assays for the Identification and Characterization of Neural Stem Cells

2.1  Introduction

2.2  Materials

2.3  Methods

2.4  Notes

Acknowledgments

References

3  Culture of Human Neural Stem Cells

3.1  Introduction

3.2  Materials

3.3  Methods

3.4  Notes

3.5  Expected Results

References

4  GMP4-Compatible Production and Expansion of Human Neural Stem Cells

4.1  Introduction

4.2  Materials

4.3  Methods

4.4  Notes

Acknowledgments

References

5  Primary Rat Neural Cultures

5.1  Introduction

5.2  Materials

5.3  Methods

5.4  Notes

References


6  Cryopreservation of Human Neural Stem and Progenitor Cells

6.1  Introduction

6.2  Materials

6.3  Methods

6.4  Notes

References

7  Cryopreservation and Recovery of Primary Rat Neural Cells

7.1  Introduction

7.2  Materials

7.3  Methods

7.4  Notes

References

8  Efficient Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent Stem Cells

8.1  Introduction

8.2  Materials

8.3  Methods

8.4  Notes

References

9  Directed Differentiation of Human NSC/NPC into Dopaminergic Neurons

9.1  Introduction

9.2  Materials

9.3  Methods

9.4  Notes

References

10  In vitro Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain Dopaminergic Neurons

10.1  Introduction

10.2  Materials

10.3  Methods

10.4  Notes

10.5  Typical Results

References

11  Generation of Astrocytes from Human Piuripotent Stem Cells using a Defined System

11.1  Introduction

11.2  Materials and Methods

11.3  Notes

References

12  Directed Differentiation of Human PSC into Oligodendrocytes

12.1  Introduction

12.2  Materials

12.3  Methods

12.4  Notes

Acknowledgments

References

13  Directed Differentiation towards Human Neural Retinal Cells

13.1  Introduction

13.2  Materials

13.3  Methods

References

14  Directed Differentiation of Photoreceptors and Retinal Pigment Epithelium from Adult Mouse Retinal Stem Cells

14.1  Introduction

14.2 Materials

14.3 Methods

14.4 Notes

Acknowledgments

References

15  Induction of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells

15.1  Introduction

15.2  Materials

15.3  Method

15.4  Notes

References

16  Neural Cell Viability Assays

16.1  Introduction

16.2  Materials

16.3  Methods

16.4  Notes

References

17  Genetic Modification of Pluripotent Stem Cell Derived Human Neural Progenitor Cells

17.1  Introduction

17.2  Materials

17.3  Methods

17.4  Notes

Acknowledgments

References

18  Electrophysiology of Neurons

18.1  Introduction

18.2  Materials

18.3  Methods

18.4 Notes

References

19  Induction and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells in Experimental Models (Rat) of Parkinson's disease

19.1  Introduction

19.2  Materials

19.3  Methods

19.4  Notes

Acknowledgments

References

20  Transplantation of Human Neural Stem Cells and Progenitors in Animal Models of Disease

20.1  Introduction

20.2  General Principles of Effective Transplantation

20.3  Different Methods of Transplantation

20.4  Materials and Method

20.5  Conclusions

20.6  Notes

Acknowledgments

References

21  Morphometric Assays for Neural Differentiation and Synaptie Development of Human iPSC-Derived NSC

21.1  Introduction

21.2  Materials

21.3  Method

21.4  Notes

Acknowledgments

References

22  BacMam Transduction of Neural Stem Cells

22.1  Introduction

22.2  Materials

22.3  Methods

22.4  Notes

Acknowledgments

References

23  The Proximity Ligation Assay: A High Throughput Technique for Protein Analysis in Neuroscience

23.1  Introduction

23.2  Materials

23.3  Methods

23.4  Notes

23.5  Troubleshooting

Acknowledgments

References

24  Quantification of Micro-RNA Expression in Human Neural Stem Cells using TaqMan Assays

24.1  Introduction

24.2  Materials

24.3  Methods

24.4  Notes

References

25  Genetic Reporter Cell Lines: Tools for Stem Cell Biology and Drug Discovery

25.1  Pluripotent Stem Cell Reporter Lines

25.2  Choice of PSC Genetic Reporter Systems

25.3  Reporter Systems

25.4  Multiple Reporter Lines

25.5  Use of Genetic Reporter Escs to Study Neural Differentiation

References

Index