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Essential guide to reading biomedical papers : recognising and interpreting best practice / editor, Phil Langton. -- Chichester, West Sussex : Wiley-Blackwell, 2013. – (61.268/E78)

Contents

    CONTENTS
    
    List of contributors ix
    Foreword xi
    Preface xiii
    Acknowledgements xvii
    Introduction xix
    Section A - Basic principles 1
    1 Philosophy of science 3
    2 Ingredients of experimental design 9
    3 Statistics: a journey that needs a guide 17
    Section B - Cell and molecular 27
    4 Organ bath pharmacology 29
    5 Small vessel myography 39
    6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49
    7 Electron microscopy (TEM and SEM) 59
    8 Fluorescence microscopy 67
    9 Intracellular 'sharp' microelectrode recording 77
    10 Single electrode voltage-clamp (SEVC) 85
    11 Patch clamp recording 95
    12 Production of antibodies 105
    13 Immunocytochemistry and immunohistochemistry 117
    14 Immunoprecipitation (IP) 129
    15 Immunoblotting (western) 137
    16 Applications of green fluorescent protein (GFP) 147
    17 Fluorescent measurement of ion activity in cells 153
    18 Detection of exocytosis -- real time 163
    19 Viral vector transgenesis 173
    20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179
    21 In situ hybridisation (ISH) 187
    22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
    23 Transcriptome analysis: microarrays 203
    24 Experimental proteomics 215
    Section C - In vivo / integrative 229
    25 Behavioural methodology 231
    26 Genetically modified mouse models 241
    27 Wireless recording of cardiovascular signals 247
    28 Electrical stimulation methods 253
    29 Extracellular recording 261
    30 Antidromic identification 271
    31 Event-triggered averaging, including spike-triggered averaging 279
    32 Axonal transport tracing of CNS pathways 285
    33 Cardiovascular methods: general considerations for human studies 291
    34 Measuring cardiac output in humans 299
    35 Measuring peripheral blood flow in humans 311
    Index 319