Essential guide to reading biomedical papers : recognising and interpreting best practice / editor, Phil Langton. -- Chichester, West Sussex : Wiley-Blackwell, 2013. – (61.268/E78) |
Contents
CONTENTS
List of contributors ix
Foreword xi
Preface xiii
Acknowledgements xvii
Introduction xix
Section A - Basic principles 1
1 Philosophy of science 3
2 Ingredients of experimental design 9
3 Statistics: a journey that needs a guide 17
Section B - Cell and molecular 27
4 Organ bath pharmacology 29
5 Small vessel myography 39
6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49
7 Electron microscopy (TEM and SEM) 59
8 Fluorescence microscopy 67
9 Intracellular 'sharp' microelectrode recording 77
10 Single electrode voltage-clamp (SEVC) 85
11 Patch clamp recording 95
12 Production of antibodies 105
13 Immunocytochemistry and immunohistochemistry 117
14 Immunoprecipitation (IP) 129
15 Immunoblotting (western) 137
16 Applications of green fluorescent protein (GFP) 147
17 Fluorescent measurement of ion activity in cells 153
18 Detection of exocytosis -- real time 163
19 Viral vector transgenesis 173
20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179
21 In situ hybridisation (ISH) 187
22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193
23 Transcriptome analysis: microarrays 203
24 Experimental proteomics 215
Section C - In vivo / integrative 229
25 Behavioural methodology 231
26 Genetically modified mouse models 241
27 Wireless recording of cardiovascular signals 247
28 Electrical stimulation methods 253
29 Extracellular recording 261
30 Antidromic identification 271
31 Event-triggered averaging, including spike-triggered averaging 279
32 Axonal transport tracing of CNS pathways 285
33 Cardiovascular methods: general considerations for human studies 291
34 Measuring cardiac output in humans 299
35 Measuring peripheral blood flow in humans 311
Index 319