 |
Lipidomics : technologies and applications / edited by Kim Ekroos. -- Weinheim : Wiley-VCH, c2012. – (58.17423/L764g) |
Contents
CONTENTS
Preface XIII
List of Contributors XV
1 Lipidomics Perspective: From Molecular Lipidomics to Validated Clinical Diagnostics 1
1.1 Introduction 1
1.2 Hierarchical Categorization of the Analytical Lipid Outputs 2
1.3 The Type of Lipid Information Delivers Different Biological Knowledge 7
1.4 Untying New Biological Evidences through Molecular Lipidomic Applications 9
1.5 Molecular Lipidomics Approaches Clinical Diagnostics 11
1.6 Current Roadblocks in Lipidomics 14
1.7 Conclusions 16
References 16
2 Lipids in Cells 21
2.1 Introduction 21
2.2 Basis of Cellular Lipid Distribution 22
2.3 Lipid Distribution by Nonvesicular Routes 23
2.4 Lipids in Different Cell Types 24
2.5 Functional Implications of Membrane Lipid Composition 26
2.6 Outlook: Collectives and Phase Separation 29
References 30
3 High-Throughput Molecular Lipidomics 35
3.1 Introduction 35
3.2 Lipid Diversity 35
3.3 Function of Molecular Lipids 38
3.4 Automated Sample Preparation 39
3.5 Different Approaches to Molecular Lipidomics 41
3.6 Data Processing and Evaluation 46
3.7 Lipidomic Workflows 47
3.8 Conclusions and Future Perspectives 48
References 49
4 Multidimensional Mass Spectrometry-Based Shotgun Lipidomics 53
4.1 Introduction 53
4.2 Multidimensional Mass Spectrometry-Based Shotgun Lipidomics 53
4.3 Application of Multidimensional Mass Spectrometry-Based Shotgun Lipidomics for Lipidomic Analysis 59
4.4 Conclusions 66
References 68
5 Targeted Lipidomics: Sphingolipidomics 73
5.1 Introduction 73
5.2 Sphingolipids Description and Nomenclature 75
5.3 Sphingolipids Analysis via Targeted LC-MS/MS 76
5.4 Applications of Sphingolipidomics in Biology and Disease 91
5.5 Conclusions 94
References 94
6 Structural Lipidomics 99
6.1 Introduction 99
6.2 Lipid Structure 100
6.3 Structural Analysis of Lipids by Mass Spectrometry 100
6.4 sn Position 105
6.5 Double Bond Position 107
6.6 Double Bond Stereochemistry 122
6.7 Conclusions 123
References 124
7 Imaging Lipids in Tissues by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry 129
7.1 Introduction 129
7.2 Sample Preparation 130
7.3 Matrix 131
7.4 Instrumentation 134
7.5 Data Processing 139
7.6 Conclusions 141
References 142
8 Lipid Informatics: From a Mass Spectrum to Interactomics 147
8.1 Introduction 147
8.2 Lipid Nomenclature 148
8.3 Basic Properties of Lipid Mass Spectrometric Data 151
8.4 Data Processing 158
8.5 Lipidomic Data Mining and Visualization 165
8.6 Lipidomic Data Integration 168
8.7 Conclusions and Future Perspectives 169
References 170
9 Lipids in Human Diseases 175
9.1 Introduction 175
9.2 Obesity 176
9.3 Dyslipidemia 177
9.4 Diabetes 177
9.5 Cardiovascular Disorders 179
9.6 Hereditary Sensory Neuropathy 181
9.7 Neurodegeneration 182
9.8 Cancer 184
9.9 Lysosomal Storage Disorders 186
9.10 Cystic Fibrosis 187
9.11 Anti-Inflammatory Lipid Mediators 188
9.12 Conclusions 188
References 189
10 Lipidomics in Lipoprotein Biology 197
10.1 Introduction 197
10.2 Metabolism of Lipoproteins 198
10.3 Lipoproteinomics in Normolipidemic Subjects 200
10.4 Altered Lipoproteinomics in Dyslipidemia 206
10.5 Conclusions 211
References 212
11 Mediator Lipidomics in Inflammation Research 219
11.1 Introduction 219
11.2 PUFA-Derived Lipid Mediators: Formation and Action 219
11.3 LC-ESI-MS/MS-Based Lipidomics 222
11.4 Mediator Lipidomics in Inflammation and Resolution 226
11.5 Conclusion and Future Perspective 230
References 230
12 Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder 233
12.1 Introduction 233
12.2 Basic Strategy of Lipidomics for Elucidating Metabolic Changes of Lipids at the Level of their Molecular Species in Metabolic Syndrome and Related Diseases 234
12.3 Analytical Systems by Mass Spectrometry in Lipidomics 235
12.4 Lipidomic Data Processing 236
12.5 Analysis of Lipids as Markers of Metabolic Syndrome 239
12.6 Direct Detection of Lipid Molecular Species in Specific Tissue Domains by Disease-Specific Changes 245
12.7 Conclusions 245
References 246
13 Lipidomics in Atherosclerotic Vascular Disease 251
13.1 Introduction 251
13.2 Lipids and Atherosclerotic Vascular Disease 253
13.3 Diagnostics and Treatment 260
13.4 Conclusions 262
References 263 ...
