Genomic imprinting : methods and protocols / edited by Nora Engel. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.925)

Contents

    Contents
    
    Contributors
    PART I PARENT-OF-ORIGIN EFFECTS
    1 Uniparental Embryos in the Study of Genomic Imprinting
    2 Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species
    3 Generation of Trophoblast Stem Cells
    4 Immunomagnetic Purification of Murine Primordial Germ Cells 61
    PART II IDENTIFYING IMPRINTED GENES
    5 Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
    6 Identification of Imprinted Loci by Transcriptome Sequencing
    7 Data Mining as a Discovery Tool for Imprinted Genes
    PART III IDENTIFYING THE REGULATORY FEATURES OF IMPRINTED DOMAINS
    8 Engineering of Large Deletions and Duplications In Vivo...
    PART IV EPIGENETICS OF IMPRINTED REGIONS
    9 Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells
    10 Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains
    11 Quantitative Chromosome Conformation Capture
    12 Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing
    PART V ANALYSIS OF IMPRINTED EXPRESSION
    13 Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies
    14 Generation of eDNA Libraries from RNP-Derived Regulatory Noncoding RNAs
    15 Co-Immunoprecipitation of Long Noncoding RNAs
    PART VI IMPRINTING IN PLANTS
    16 Specialized Technologies for Epigenetics in Plants
    PART VII EVOLUTION OF IMPRINTED GENES
    17 Computational Studies of Imprinted Genes...
    18 Insights on Imprinting from Beyond Mice and Men
    19 Nonmammalian Parent-of-Origin Effects
    Index