Plant signalling networks : methods and protocols / edited by Zhi-Yong Wang, Zhenbiao Yang. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.876)

Contents

    Contents
    
    Preface
    Contributors
    1 Experimental Analysis of Receptor Kinase Phosphorylation
    2 Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis and Functional Phosphoproteomic Strategies
    3 Identification of O-linked β-D-N-acetylglucosamine-Modified Proteins from Arabidopsis
    4 Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis
    5 Quantitative Analysis of Plasma Membrane Proteome Using Two-Dimensional Difference Gel Electrophoresis
    6 Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana
    7 Small-Molecule Dissection of Brassinosteroid Signaling
    8 A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination
    9 Activation Tagging
    10 Rho GTPase Activity Analysis in Plant Cells
    11 Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy
    12 In Vivo Ubiquitination Assay by Agroinfiltration
    13 In Vitro Protein Ubiquitination Assay
    14 Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)
    15 Smart Pooling of mRNA Samples for Efficient Transcript Profiling
    16 Transient Expression Assays for Quantifying Signaling Output
    17 Genome-Wide Profiling of Uncapped mRNA
    18 Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches
    Index