RNA abundance analysis : methods and protocols / edited by Hailing Jin, Walter Gassmann. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.883) |
Contents
Contents
Contributors
1 SuperSAGE: Powerful Serial Analysis of Gene Expression
2 Northern Blot Analysis for Expression Profiling of mRNAs and Small RNAs
3 Construction of RNA-Seq Libraries from Large and Microscopic Tissues for the Illumina Sequencing Platform
4 Strand-Specific RNA-Seq Applied to Malaria Samples
5 RNA In Situ Hybridization in Arabidopsis
6 Laser Microdissection of Cells and Isolation of High-Quality
RNA After Cryosectioning
7 Detection and Quantification of Alternative Splicing Variants Using RNA-seq
8 Separating and Analyzing Sulfur-Containing RNAs with Organomercury Gels 111
9 RNAse Mapping and Quantitation of RNA Isoforms 121
10 Detection and Quantification of Viral and Satellite RNAs in Plant Hosts 131
11 In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes
12 Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora and Fusarium Using Illumina High-Throughput Deep Sequencing
13 Isolation and Profiling of Protein-Associated Small RNAs
14 New Virus Discovery by Deep Sequencing of Small KNAs
15 Global Assembly of Expressed Sequence Tags 193
16 ComputationalAnalysis of RNA-seq 201
17 Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from SmalI-RNA Deep Sequencing Data 221
Index