Antiviral resistance in plants : methods and protocols / edited by John M. Watson and Ming-Bo Wang. — New York : Humana Press, c2012. – (58.17/M592/v.894)

Contents

        CONTENTS
    
    Contributors
    1 A Historical Overview of RNAi in Plants
    2 RNA Silencing and Antiviral Defense in Plants
    3 Biological Chemistry of Virus-Encoded Suppressors of R.NA Silencing An Overview
    4 Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules
    5 Strategies for Viral Cross Protection in Plants
    6 Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing
    7 Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors
    8 Characterization of Plant Virus-Encoded Gene Silencing Suppressors 113
    9 Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing
    10 Modeling Antiviral Resistance in Plants
    11 Isolation and Detection of Small RNAs from Plant Tissues
    12 Isolation and Analysis of Small RNAs from Virus-Infected Plants
    13 Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance
    14 Artificial MicroRNAs for Plant Virus Resistance
    15 Isolation and Biochemical Analysis of Plant Small RNAs
    16 Isolation and Analysis of Plant RNA-Dependent RNA Polymerases 241
    17 Plant Viroids: Isolation， Characterization/Detection， and Analysis
    18 Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing
    19 Virus-Induced Gene Silencing in Soybean
    20 Chimeric， Infectious， and Stable Virus Transcripts to Study RNA Silencing in "Dark Green" Islands
    21 Rolling Circle Amplification-Mediated Long Hairpin RNA Library Construction in Plants 309
    22 The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing
    23 Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus Replication 345
    24 Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections
    Index