Human embryonic stem cells handbook / edited by Kursad Turksen. — New York : Humana Press, c2012. – (58.17/M592/v.873)

Contents

        Contents
    
    Preface
    Contributors
    1 Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology 1
    2 Human Embryonic Stem Cells Derived in Xeno-Free Conditions 13
    3 Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense， Denmark 33
    4 Principles for Derivation of Human Embryonic Stem Cells 53
    5 Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells 69
    6 Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos 81
    7 Analysis of LINE-1 Expression in Human Pluripotent Cells 113
    8 Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan 127
    9 Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos 151
    10 Derivation， Expansion， and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos 163
    11 Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders
    12 Single-Cell Enzymatic Dissociation of hESC Lines OxF1-OxF4 and Culture in Feeder-Free Conditions 209
    13 Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension 217
    14 Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells 227
    15 Derivation， Propagation， and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)
    16 Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol 247
    17 Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells 261
    18 Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells 269
    19 Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next-Generation Sequencing 281
    20 Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis 297
    Index 307