Single-stranded DNA binding proteins : methods and protocols / edited by James L. Keck. — New York : Humana Press, 2012. – (58.17/M592/v.922)

Contents

    Contents
    
    Contributors
    1 Functions of Single-Strand DNA-Binding Proteins in DNA Replication， Recombination， and Repair
    2 Structural Diversity Based on Variability in Quaternary Association A Case Study Involving Eubacterial and Related SSBs
    3 SSB Binding to ssDNA Using Isothermal Titration Calorimetry
    4 SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy
    5 Single-Molecule Analysis of SSB Dynamics on Single-Stranded DNA 85
    6 Sample Preparation Methods to Analyze DNA-Induced Structural Changes in Replication Protein A 101
    7 Structural Studies of SSB Interaction with RecoO 123
    8 Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation 133
    9 Ammonium Sulfate Co-precipitation of SSB and Interacting Proteins 151
    10 Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy
    11 Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins 161
    12 Methods for Analysis of SSB-Protein Interactions by SPR
    13 Use of Native Gels to Measure Protein Binding to SSB
    14 Identification of Small Molecules That Disrupt SSB-Protein Interactions Using a High-Throughput Screen
    15 Detection of Posttranslational Modifications of Replication Protein A
    16 Detecting Posttranslational Modifications of Bacterial SSB Proteins
    17 Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA
    18 Fluorescent Single-Stranded DNA-Binding Proteins Enable In Vitro and In Vivo Studies
    19 Use of Fluorescently Tagged SSB Proteins in In Vivo Ix3calization Experiments
    Index