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Embryo culture : methods and protocols / edited by Gary D. Smith, Jason E. Swain, Thomas B. Pool. — New York : Humana Press, c2012. – (58.17/M592/v.912)

Contents

    Contents
    
    Contributors
    1 Historical Background of Gamete and Embryo Culture
    2 Utility of Animal Models for Human Embryo Culture Development: Rodents
    3 Utility of Animal Models for Human Embryo Culture Development: Domestic Species
    4 Utility of Animal Models for Human Embryo Culture Nonhuman Primates
    5 Media Compulsion: Salts and Osmolality
    6 Media Composition: Energy Sources and Metabolism
    7 Media Composition: Amino Acids and Cellular Homeostasis
    8 Media Composition: Macromolecules and Embryo Growth
    9 Media Composition: Antioxidants/Chelators and Cellular Function
    10 Media Composition: pH and Buffers
    11 Media Composition: Growth Factors
    12 Culture Systems: Single Step
    13 Culture Systems: Sequential
    14 Culture Systems: Embryo Co-Culture
    15 Culture Systems: Low-Oxygen Culture
    16 Culture Systems: Embryo Density
    17 Culture Systems: Air Quality
    18 Culture Systems: Mineral Oil Overlay
    19 Culture Systems: Physiological and Environmental Factors That Can Affect the Outcome of Human ART
    20 Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
    21 Human Embryo Culture Media Comparisons
    22 Culture Systems: Embryo Culture and Monozygotic Twinning
    23 Embryo Culture and Epigenetics
    Index 423