Plant salt tolerance : methods and protocols / edited by Sergey Shabala, Tracey Ann Cuin. — New York : Humana Press ;Springer, c2012. – (58.17/M592/v.913) |
Contents
Contents
Preface
Contributors
PART I MICROELECTRODE TECHNIQUES
1 Patch-Clamp Protocols to Study Cell Ionic Homeostasis Under Saline Conditions
2 Studying Plant Salt Tolerance with the Voltage Clamp Technique 19
3 Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions 35
4 Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes 67
5 Single-Cell Sampling and Analysis (SiCSA) 79
6 Non-invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems 101
7 Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-invasive Microelectrode Measuring MIFE Technique 119
PART II IMAGING TECHNIQUES
8 Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt 137
9 Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research 149
10 Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress 163
11 Infrared Thermography in Plant Phenotyping for Salinity Tolerance 173
12 In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy 191
PART III BIOCHEMICAL ASSAYS
13 Metabolomics for Salinity Research 203
14 Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping 217
15 Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues 225
16 Quantification of the Antioxidant Activity in Salt-Stressed Tissues 237
17 Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues 251
PART IV MOLECULAR TECHNIQUES
18 Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Specific Responses to Salinity Stress in Arabidopsis and Rice 265
19 Transformation Using Controlled cDNA Overexpression System 277
20 Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress 291
21 Marker-Assisted Selection in Plant Breeding for Salinity Tolerance 305
22 Transcriptomics on Small Samples 335
23 Plastid Transformation for Abiotic Stress Tolerance in Plants 351
24 Manipulating Expression of Tonoplast Transporters 359
25 Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities 371
PART V OTHER METHODS
26 Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions 389
27 Trait Dissection of Salinity Tolerance with Plant Phenomics 399
28 Measuring Soil Salinity 415
Index 427