Correlative light and electron microscopy / edited by Thomas Muller-Reichert [and] Paul Verkade. — Amsterdam, Netherlands ;Boston, Mass. : Elsevier/Academic Press, 2012. – (58.1574/M592/v.111) |
Contents
CONTENTS
Contributors
Preface
1. Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
Acknowledgments
References
2. Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy
I. Introduction and Rationale
II. Materials
III. Methods
IV. Discussion
V. Summary
Acknowledgments
References
3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
Abstract
I. Introduction
II. Correlative Microscopy: Reporter Systems
III. Correlative Microscopy of Tissues
IV. Conclusions
References
4. Correlative Light and Electron Microscopy in Parasite Research
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Experiments and Materials
V. Results and Discussion
VI. Summary
Acknowledgments
References
5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Summary and Outlook
Acknowledgments
References
6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
Abstract
I. Introduction
II. Materials
III. Methods
IV. Notes
Acknowledgments
References
7. Correlative Light and Electron Microscopy of GFP
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
Acknowledgments
References
8. Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
Abstract
I. Introduction
II. The Crowded Cell and Spatiotemporal Proteomics
III. What EM Has to Offer
IV. Immunomarkers
V. Genetically Appended or Inserted Protein Tags
VI. Types of Genetic Tags Currently Available
VII. Future Directions and Challenges
Acknowledgments
References
9. Correlated Light Microscopy and Electron Microscopy
Abstract
Abbreviations
I. Introduction
II. Correlated Light and Electron Microscopy: Using the Best of Two Worlds
III. ROIs: Search & Find Tools
IV. Our Approach: Virtual Reality Overlay During Preparation
V. Discussion and Conclusion
VI. Future Perspective
Acknowledgments
References
10. Capturing Endocytic Segregation Events with HPF-CLEM
Abstract
I. Introduction
I1. Methods
III. Outlook
Acknowledgments
References
11. Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
Acknowledgments
References
12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo
Abstract
I. Introduction
II. Methods
III. Instrumentation and Material
IV. Discussion
Acknowledgments
References
13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
14. Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion and Outlook
Acknowledgments
References
15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Instrumentations and Materials
V. Discussion
VI. Perspective
Acknowledgments
References
16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
Abstract
I. Introduction: Atmospheric Scanning Electron Microscopy
II. Design of the JEOL JASM-6200
III. Sample Preparation
IV. Application Studies
V. Discussion
Acknowledgments
References
17. Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures
Abstract
I. Introduction
II. Rationale
III. Methods
IV. Materials and Instrumentation
V. Discussion
VI. Summary
Acknowledgments
References
18. Correlative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
Abstract
I. Introduction
II. Rationale
III. Materials
IV. Methods
V. Discussion
VI. Summary
Acknowledgments
References
Index
Volumes in Series