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Correlative light and electron microscopy / edited by Thomas Muller-Reichert [and] Paul Verkade. — Amsterdam, Netherlands ;Boston, Mass. : Elsevier/Academic Press, 2012. – (58.1574/M592/v.111)

Contents

    CONTENTS
    
    Contributors
    Preface
    1. Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    Acknowledgments
    References
    2. Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy
    I. Introduction and Rationale
    II. Materials
    III. Methods
    IV. Discussion
    V. Summary
    Acknowledgments
    References
    3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    Abstract
    I. Introduction
    II. Correlative Microscopy: Reporter Systems
    III. Correlative Microscopy of Tissues
    IV. Conclusions
    References
    4. Correlative Light and Electron Microscopy in Parasite Research
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Experiments and Materials
    V. Results and Discussion
    VI. Summary
    Acknowledgments
    References
    5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Summary and Outlook
    Acknowledgments
    References
    6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    Abstract
    I. Introduction
    II. Materials
    III. Methods
    IV. Notes
    Acknowledgments
    References
    7. Correlative Light and Electron Microscopy of GFP
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Discussion
    Acknowledgments
    References
    8. Picking Faces out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    Abstract
    I. Introduction
    II. The Crowded Cell and Spatiotemporal Proteomics
    III. What EM Has to Offer
    IV. Immunomarkers
    V. Genetically Appended or Inserted Protein Tags
    VI. Types of Genetic Tags Currently Available
    VII. Future Directions and Challenges
    Acknowledgments
    References
    9. Correlated Light Microscopy and Electron Microscopy
    Abstract
    Abbreviations
    I. Introduction
    II. Correlated Light and Electron Microscopy: Using the Best of Two Worlds
    III. ROIs: Search & Find Tools
    IV. Our Approach: Virtual Reality Overlay During Preparation
    V. Discussion and Conclusion
    VI. Future Perspective
    Acknowledgments
    References
    10. Capturing Endocytic Segregation Events with HPF-CLEM
    Abstract
    I. Introduction
    I1. Methods
    III. Outlook
    Acknowledgments
    References
    11. Targeted Ultramicrotomy: A Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    Acknowledgments
    References
    12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo
    Abstract
    I. Introduction
    II. Methods
    III. Instrumentation and Material
    IV. Discussion
    Acknowledgments
    References
    13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    References
    14. Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion and Outlook
    Acknowledgments
    References
    15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentations and Materials
    V. Discussion
    VI. Perspective
    Acknowledgments
    References
    16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
    Abstract
    I. Introduction: Atmospheric Scanning Electron Microscopy
    II. Design of the JEOL JASM-6200
    III. Sample Preparation
    IV. Application Studies
    V. Discussion
    Acknowledgments
    References
    17. Bridging Microscopes: 3D Correlative Light and Scanning Electron Microscopy of Complex Biological Structures
    Abstract
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials and Instrumentation
    V. Discussion
    VI. Summary
    Acknowledgments
    References
    18. Correlative Light and Volume Electron Microscopy: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
    Abstract
    I. Introduction
    II. Rationale
    III. Materials
    IV. Methods
    V. Discussion
    VI. Summary
    Acknowledgments
    References
    Index
    Volumes in Series