Gene synthesis : methods and protocols / edited by Jean Peccoud. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.852)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I ASSEMBLY OF OLIGONUCLEOTIDES IN SYNTHONS
    1 Building Block Synthesis Using the Polymerase Chain Assembly Method
    2 Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments
    3 TopDown Real-Time Gene Synthesis
    4 De Novo DNA Synthesis Using Single-Molecule PCR
    PART II SYNTHON ASSEMBLY
    5 SLIC: A Method for Sequence- and Ligation-Independent Cloning
    6 Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli
    7 Assembling DNA Fragments by USER Fusion
    8 Fusion PCR via Novel Overlap Sequences
    9 Using Recombineering to Generate Point Mutations: The Oligonucleotide-Based "Hit and Fix" Method 111
    10 Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method 121
    11 Assembling Large DNA Segments in Yeast 133
    12 Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides
    13 Cloning Whole Bacterial Genomes in Yeast
    14 Production of Infectious Poliovirus from Synthetic Viral Genomes
    PART III SOFTWARE FOR GENE SYNTHESIS
    15 In Silico Design of Functional DNA Constructs 197
    16 Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis
    17 De Novo Gene Synthesis Design Using TmPrime Software
    18 Design-A-Gene with GeneDesign 235
    PART IV EDUCATION AND SECURITY
    19 Leading a Successful iGEM Team
    20 The Build-a-Genome Course
    21 DNA Synthesis Security
    Index 297