Single-cell analysis : methods and protocols / edited by Sara Lindstrom, Helene Andersson-Svahn. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.853)

Contents

    Contents
    
    Preface
    Contributors
    1 Introduction: Why Analyze Single Cells?
    PART I CONVENTIONAL METHODS
    2 Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview 13
    3 Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry 17
    4 Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts
    PART II MINIATURIZED SOLUTIONS
    5 Single-Cell Culture in Microwells 41
    6 Isodielectric Separation and Analysis of Cells 53
    7 Single Cell Electroporation Using Microfluidic Devices
    8 Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
    9 Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
    10 Droplet Microfluidics for Single-Cell Analysis I05
    11 Screening of Antigen-Specific Antibody-Secreting Cells 141
    12 Analysis of Single Eukaryotic Cells Using Raman Tweezers
    13 Single-Cell Microinjection Technologies
    14 Ultrasonic Manipulation of Single Cells
    PART III REVIEWS ON CHOSEN SUBJECTS WITHIN THE FIELD
    15 Expanding the Horizons for Single-Cell Applications on Lab-on-a-Chip Devices
    16 Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
    Index