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Host-fungus interactions : methods and protocols / edited by Alexandra C. Brand, Donna M. MacCallum. — New York : Humana Press : Springer, c2012. – (58.17/M592/v.845) |
Contents
Contents
Preface
Contributors
PART I GENE DISRUPTION
1 Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy 3
2 Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans
3 Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans
4 Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatum
5 Targeted Gene Disruption in Cryptococcus neoformans Using Double-Joint PCR with Split Dominant Selectable Markers 67
6 Multiple Gene Deletion in Cryptococcus neoformans Using the Cre-lox System
7 Gene Disruption in Aspergillusfumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast
8 Targeted Gene Deletion in Aspergillusfumigatus Using the Hygromycin-Resistance Split-Marker Approach
9 Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers
PART II MODULATION OF GENE EXPRESSION: RNAI GENE KNOCKDOWN
10 RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum
11 RNA Interference in Cryptococcus neoformans 165
12 Gene Knockdown in Paracoccidioides brasiliensis Using Antisense RNA
PART III MODULATION OF GENE EXPRESSION: REGULATABLE PROMOTERS
13 Tetracycline-Inducible Gene Expression in Candida albicans 201
14 Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii 211
15 Modular Gene Over-expression Strategies for Candida albicans 227
PART IV HOST RESPONSES TO INFECTION IN VITRO
16 Interactions Between Macrophages and Cell Wall Oligosaccharides of Candida albicans 247
17 Murine Bone Marrow-Derived Dendritic Cells and T-Cell Activation by Candida albicans 261
18 Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils 277
19 Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions 289
20 Simple Assays for Measuring Innate Interactions with Fungi 303
21 Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells 319
22 Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium 333
23 Analysis of Host-Cell Responses by Immunoblotting， ELISA， and Real-Time PCR
24 In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus
25 Biofilm Formation Studies in Microtiter Plate Format
PART V FUNGAL RESPONSES DURING INFECTION
26 Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection 381
27 Laser Capture Microdissection of Candida albicans from Host Tissue 397
28 Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples 411
PART VI HOST RESPONSES "FO INFECTION IN VIVO
29 Cytokine Measurement Using Cytometric Bead Arrays 425
30 Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis 435
PART VII NON-MAMMALIAN MODEL SYSTEMS FOR HOST--FUNGAL INTERACTIONS
31 Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi 447
32 Drosophila melanogaster as a Model Organism for Invasive Aspergillosis 455
33 Galleria mellonella as a Model for Fungal Pathogenicity Testing 469
34 Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi 487
PART VIII MAMMALIAN HOSTS AS INFECTION MODELS
35 Mouse Intravenous Challenge Models and Applications 499
36 A Nebulized Intra-tracheal Rat Model of Invasive Pulmonary Aspergillosis
37 Invasive Models of Histoplasmosis 519
38 Murine Model of Concurrent Oral and Vaginal Candida albicans Colonisation
39 A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections
40 Modeling of Fungal Biofilms Using a Rat Central Vein Catheter
41 Orogastrointestinal Model of Mucosal and Disseminated Candidiasis 557
42 A Nonlethal Murine Cutaneous Model of Invasive Aspergillosis
Index
