Neurotrophic factors : methods and protocols / edited by Stephen D. Skaper. — New York : Humana Press, c2012. – (58.17/M592/v.846) |
Contents
Contents
Preface
Contributors
1 The Neurotrophin Family of Neurotrophic Factors: An Overview
2 Neuronal Growth-Promoting and Inhibitory Cues in Neuroprotection and Neuroregeneration 13
3 Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents
4 Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum
5 Culture of Rodent Cortical and Hippocampal Neurons 49
6 Amyloid 13-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons
7 Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord 67
8 Central Nervous System Neuron-Glia Co-culture Models 79
9 Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons 91
10 Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions 103
11 Rodent Retinal Ganglion Cell Cultures 117
12 Culture of Purified Glial Cell Populations from Optic Nerve 131
13 Isolation and Culture of Rat Cone Photoreceptor Cells 147
14 Culture of Rat Retina Pigmented Epithelial Cells
15 Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms That Underlie Axon Growth, Guidance and Regeneration
16 Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats
17 Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man 189
18 Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson's Disease
19 Compartmented Chambers for Studying Neurotrophic Factor Action
20 Preparation and Culture of Adrenal Chromaffin Cells
21 Indirect Immunofluorescence Staining of Cultured Neural Cells
22 Neurite Outgrowth Assessment Using High Content Analysis Methodology
23 Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth
24 High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures
25 Imaging Amyloid Precursor Protein In Vivo: An Axonal Transport Assay
26 The Use of Specific AAV Serotypes to Stably Transduce Primary CNS Neuron Cultures 305
27 Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides 321
28 [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells 333
29 Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents 343
30 A 6-Hydroxydopamine In Vivo Model of Parkinson's Disease 355
31 Brain Microdialysis in Freely Moving Animals 365
32 Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion 383
33 Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection
Index 417