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Neurotrophic factors : methods and protocols / edited by Stephen D. Skaper. — New York : Humana Press, c2012. – (58.17/M592/v.846)

Contents

    Contents
    
    Preface
    Contributors
    1 The Neurotrophin Family of Neurotrophic Factors: An Overview
    2 Neuronal Growth-Promoting and Inhibitory Cues in Neuroprotection and Neuroregeneration 13
    3 Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents
    4 Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum
    5 Culture of Rodent Cortical and Hippocampal Neurons 49
    6 Amyloid 13-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons
    7 Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord 67
    8 Central Nervous System Neuron-Glia Co-culture Models 79
    9 Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons 91
    10 Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions 103
    11 Rodent Retinal Ganglion Cell Cultures 117
    12 Culture of Purified Glial Cell Populations from Optic Nerve 131
    13 Isolation and Culture of Rat Cone Photoreceptor Cells 147
    14 Culture of Rat Retina Pigmented Epithelial Cells
    15 Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms That Underlie Axon Growth, Guidance and Regeneration
    16 Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats
    17 Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man 189
    18 Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson's Disease
    19 Compartmented Chambers for Studying Neurotrophic Factor Action
    20 Preparation and Culture of Adrenal Chromaffin Cells
    21 Indirect Immunofluorescence Staining of Cultured Neural Cells
    22 Neurite Outgrowth Assessment Using High Content Analysis Methodology
    23 Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth
    24 High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures
    25 Imaging Amyloid Precursor Protein In Vivo: An Axonal Transport Assay
    26 The Use of Specific AAV Serotypes to Stably Transduce Primary CNS Neuron Cultures 305
    27 Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides 321
    28 [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells 333
    29 Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents 343
    30 A 6-Hydroxydopamine In Vivo Model of Parkinson's Disease 355
    31 Brain Microdialysis in Freely Moving Animals 365
    32 Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion 383
    33 Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection
    Index 417