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Handbook of molecular and cellular methods in biology and medicine / edited by Leland J. Cseke ... [et al.]. — 3rd ed. — Boca Raton : CRC Press/Taylor & Francis Group, 2011. – (58.178073/H236/3rd ed.) |
Contents
Contents
Preface
Editors
Contributors
PART I DNA-Based Technology
Chapter 1 Isolation and Purification of DNA 3
Chapter 2 DNA Cloning Strategies 29
Chapter 3 Polymerase Chain Reaction Methodologies 37
Chapter 4 Preparation of Nucleic Acid Probes 57
Chapter 5 Southern Blot Hybridization 77
Chapter 6 Genomic DNA Libraries 97
Chapter 7 DNA Sequencing and Analysis 141
PART II RNA-Based Technology
Chapter 8 Isolation and Purification of RNA 167
Chapter 9 Real-Time PCR and qRT-PCR Methodologies 187
Chapter 10 Preparation of RNA Probes 197
Chapter 11 Northern Blot Hybridization 211
Chapter 12 cDNA Libraries 217
Chapter 13 Differential Display 261
Chapter 14 Localization of RNA Expression 273
PART III Protein-Based Technology
Chapter 15 Isolation and Purification of Proteins 291
Chapter 16 Western Blot Hybridization 313
Chapter 17 Localization of Protein Expression 331
Chapter 18 DNA Footprinting and Gel Retardation Assay 337
Chapter 19 Protein-Protein and Protein-Ligand Interactions 351
PART IV Metabolites
Chapter 20 Isolation and Purification of Metabolites 367
Chapter 21 Analysis of Metabolites 381
PART V Genomics， Proteomics， and Metabolomics
Chapter 22 Bioinformatics and Data Analysis 409
Chapter 23 Microarray Platforms 437
Chapter 24 Microarray Data Collection 447
Chapter 25 Microarray Data Analysis 455
Chapter 26 Proteomics: Data Collection and Analysis 459
Chapter 27 Metabolomics: Data Collection and Analysis 471
Chapter 28 Common Protocols on Membrane Lipid Analysis and Lipidomics 485
Chapter 29 Integrated Functional Genomics 509
PART VI Manipulation of Biological Systems
Chapter 30 Inhibition of Gene Expression 521
Chapter 31 Inhibition of Protein Synthesis 549
Chapter 32 Gene Transfer and Expression in Animal Cells 557
Chapter 33 Methods of Stem Cell Lineage Analysis In Situ and in Cell Culture 579
Chapter 34 Tissue Engineering of Fully Differentiated Tissue: Lessons from Work on Cardiac Myocytes 593
Chapter 35 Plant Tissue and Cell Culture 607
Chapter 36 Gene Transfer and Expression in Plants 629
PART VII Macromolecular Analyses
Chapter 37 Microscopy: Light and Confocal 657
Chapter 38 Microscopy: Scanning Electron， Environmental Scanning Electron， and Transmission Electron 667
Chapter 39 Laser Capture Microdissection and Whole Genome Amplification 687
Index 701
