Primary and stem cells : gene transfer technologies and applications / edited by Uma Lakshmipathy and Bhaskar Thyagarajan. — Hoboken, N.J. : John Wiley and Sons, c2012. – (58.22/P952)

Contents

    CONTENTS
    
    FOREWORD
    PREFACE
    CONTRIBUTORS
    PART I: CLONING AND GENE DELIVERY
    1. DNA Assembly Technologies Based on Homologous Recombination
    2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells
    3. Strategies for the Delivery of Naked DNA
    PART II NONINTEGRATING TECHNOLOGIES
    4. Episomal Vectors
    5. Nonintegrating DNA Virus
    6. Nonintegrating RNA Viruses
    7. Protein Delivery
    PART III: INTEGRATING TECHNOLOGIES
    8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery
    9. Integrating Viral Vectors for Gene Modifications
    10. Bacteriophage Integrases for Site-Specific Integration
    11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells
    PART IV: APPLICATIONS
    12. Modified Stem Cells as Disease Models and in Toxicology Screening
    13. Screening and Drug Discovery
    INDEX