Next-generation MicroRNA expression profiling technology : methods and protocols / edited by Jian-Bing Fan. — New York : Humana Press, c2012. – (58.17/M592/v.822)

Contents

    Contents
    
    Preface
    Contributors
    1 Controlling miRNA Regulation in Disease
    2 Introduction to miRNA Profiling Technologies and Cross-Platform Comparison
    3 Stem-Loop RT-qPCR for MicroRNA Expression Profiling
    4 Poly(T) Adaptor RT-PCR
    5 MicroRNA In Situ Hybridization
    6 Agilent MicroRNA Microarray Profiling System
    7 miRNA Expression Profiling Using Illumina Universal BeadChips 103
    8 MicroRNA Expression Analysis Using the Affymetrix Platform 117
    9 Individualized miRNA Assay Panels Using Optically Encoded Beads 131
    10 Microfluidic Primer Extension Assay
    11 MicroRNA Profiling Using μParaflo Microfluidic Array Technology 153
    12 MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform 183
    13 Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application 189
    14 Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing 205
    15 Profiling of Short RNAs Using Helicos Single-Molecule Sequencing 219
    16 deepBase: Annotation and Discovery of MicroRNAs and Other Noncoding RNAs from Deep-Sequencing Data
    17 PhenomiR: MicroRNAs in Human Diseases and Biological Processes
    18 miRNA Expression Profiling: From Reference Genes to Global Mean Normalization
    19 miRNA Data Analysis: Next-Gen Sequencing
    20 Integrated miRNA Expression Analysis and Target Prediction
    21 miRNAs in Human Cancer
    22 Blood-Based miRNA Preparation for Noninvasive Biomarker Development
    Index 339