Cytokine protocols / edited by Marc de Ley. — 2nd ed. — New York : Humana Press, c2012. – (58.17/M592/v.820)

Contents

    Contents
    
    Preface
    Contributors ix
    1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods Can Engender Discovery 1
    2 The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels
    3 Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles Using Affymetrix Microarray and DAVID 25
    4 Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues 55
    5 Evaluating Posttranscriptional Regulation of Cytokine Genes
    6 Cloning of Cytokine 3" Untranslated Regions and Posttranscriptional Assessment Using Cell-Based GFP Assay 91
    7 Integrin-Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs In Vitro and In Vivo 105
    8 Hammerhead Ribozyme-Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor 117
    9 Control of the Interferon Response in RNAi Experiments 133
    10 shRNA-Induced Interferon-Stimulated Gene Analysis
    11 Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells 179
    12 Ligand Affinity Chromatography， an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins 195
    13 In Vitro Stimulation and Detection of IFNα Production in Human Plasmacytoid Dendritic Cells
    14 A Sensitive and Versatile Cytokine Bioassay Based on Type I Interferon Signaling in 2fTGH Cells 231
    Index