Plant metabolomics : methods and protocols / edited by Nigel W. Hardy, Robert D. Hall. — New York, N.Y. : Humana Press, c2012. – (58.17/M592/v.860) |
Contents
Contents
Preface
Contributors
1 Practical Applications of Metabolomics in Plant Biology
PART I MATERIAL PREPARATION
2 Aspects of Experimental Design for Plant Metabolomics Experiments and Guidelines for Growth of Plant Material 13
3 Separating the Inseparable: The Metabolomic Analysis of Plant-Pathogen Interactions 31
4 Precautions for Harvest, Sampling, Storage, and Transport of Crop Plant Metabolomics Samples 51
5 Tissue Preparation Using Arabidopsis
PART II CHEMICAL ANALYSIS APPROACHES
6 Solid Phase Micro-Extraction GC-MS Analysis of Natural Volatile Components in Melon and Rice
7 Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass Spectrometry
8 High-Performance Liquid Chromatography-Mass Spectrometry Analysis of Plant Metabolites in Brassicaceae
9 UPLC-MS-Based Metabolite Analysis in Tomato
10 High Precision Measurement and Fragmentation Analysis for Metabolite Identification 145
11 Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Plant Metabolite Profiling and Metabolite Identification
12 Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics 177
13 ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in Cereal Grains
14 The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses 213
PART III DATA ANALYSTS
15 Data (Pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data Using MetAlign.
16 TagFinder: Preprocessing Software for the Fingerprinting and the Profiling of Gas Chromatography-Mass Spectrometry Based Metabolome Analyses
17 Chemical Identification Strategies Using Liquid Chromatography-Photodiode Array-Solid-Phase Extraction-Nuclear Magnetic Resonance/Mass Spectrometry 287
18 A Strategy for Selecting Data Mining Techniques in Metabolomics 317
Index