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Proteoglycans : methods and protocols / edited by Francoise Redini. — New York : Humana Press, c2012. – (58.17/M592/v.836) |
Contents
Contents
Dedication
Preface
Contributors
PART I PROTEOGLYCANS
1 Proteoglycans: Gene Cloning 3
2 Proteoglycan: Site Mapping and Site-Directed Mutagenesis 23
3 Mapping of the Wnt/J3-Catenin/TCF Response Elements in the Human Versican Promoter 35
4 Gene Silencing in Mouse Embryonic Stem Cells 53
5 A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout 63
6 Detection of Neurocan in Cerebrospinal Fluid 87
PART II GLYCOSAMINOGLYCANS ANALYSIS
7 Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization) 99
8 Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis
9 Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography， Capillary Electrophoresis， and Specific Labeling 131
10 Brain Chondroitin/Dermatan Sulfate， from Cerebral Tissue to Fine Structure: Extraction， Preparation， and Fully Automated Chip-Electrospray Mass Spectrometric Analysis 145
11 Use of Neutrons Reveals the Dynamics of Cell Surface Glycosaminoglycans 161
12 Following Protein-Glycosaminoglycan Polysaccharide Interactions with Differential Scanning Fluorimetry 171
13 In Vivo Scintigraphic Imaging of Proteoglycans 183
PART III PGs: MULTIFUNCTIONAL CELL REGULATORS
14 Serglycin: The Master of the Mast Cell 201
15 Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry
16 Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors 239
PART IV PROTEOGLYCANS INVOLVEMENT IN PATHOPHYSIOLOGY
17 Models for Studies of Proteoglycans in Kidney Pathophysiology 259
18 Lumican Promotes Corneal Epithelial Wound Healing 285
19 Shedding of Cell Membrane-Bound Proteoglycans 291
20 Modulatory Effects of Proteoglycans on Proteinase Activities 307
21 Proteoglycans and Osteolysis 323
22 Proteoglycans and Cartilage Repair 339
Index
