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Recombinant gene expression : reviews and protocols / edited by Argelia Lorence. — 3rd ed. — New York : Humana Press, c2012. – (58.17/M592/v.824)

Contents

    Contents
    
    Contributors
    PART I GENERAL ASPECTS
    1 Using Folding Promoting Agents in Recombinant Protein Production: A Review
    2 Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry
    3 A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression
    4 Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins
    PART II PROKARYOTES
    5 Heat-Shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli 109
    6 The Use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella 131
    7 Optimization of Purification Protocols Based on the Step-by-Step Monitoring of the Protein Aggregates in Soluble Fractions 145
    8 Heterologous Protein Expression by Lactococcus lactis 155
    9 An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview 167
    10 Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
    11 A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125
    12 A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-Protein Interaction
    13 Heterologous High-Level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus
    14 Plasmid DNA Production for Therapeutic Applications
    PART III LOWER EUKARYOTES
    15 Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review
    16 Expression of Multisubunit Proteins in Leishmania tarentolae
    PART IV FUNGI
    17 Recombinant Protein Production in Yeasts
    18 Yeasts as a Tool for Heterologous Gene Expression
    19 The Cre/Lox System: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes
    20 Aptamer-Regulated Expression of Essential Genes in Yeast
    21 Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
    22 A Thiamine-Regulatable Epitope-Tagged Protein Expression System in Fission Yeast
    23 Heterologous Gene Expression by Chromosomal Integration in Fission Yeast 433
    24 Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae 451
    PART V PLANTS AND PLANT CELLS
    25 Recombinant Protein Production in Plants: Challenges and Solutions 469
    26 A Novel Plant Cell Bioproduction Platfbrm for High-Yield Secretion of Recombinant Proteins 483
    27 Super-promoter: TEV, a Powerful Gene Expression System for Tobacco Hairy Roots
    28 Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology 527
    29 Quality Assessment of Recombinant Proteins Produced in Plants 535
    30 Cell-Free Protein Synthesis as a Promising Expression System for Recombinant Proteins 565
    PART VI ANIMALS AND ANIMAL CELLS
    31 The Use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines 581
    32 Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity 595
    33 High-Throughput Baculovirus Expression in Insect Cells 609
    34 Recombinant Protein Expression in Milk of Livestock Species 629
    Index 643