Platelets and megakaryocytes. Volume 3, Additional protocols and perspectives / edited by Jonathan M. Gibbins, Martyn P. Mahaut-Smith. — New York, NY : Humana Press, c2012. – (58.17/M592/v.788)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I ADDITIONAL PROTOCOLS FOR THE STUDY OF PLATELET FUNCTION
    1 Intravital Imaging of Thrombus Formation in Small and Large Mouse Arteries: Experimentally Induced Vascular Damage and Plaque Rupture In Vivo
    2 Assessment of Platelet Aggregation Responses In Vivo in the Mouse
    3 Focal Cerebral Ischemia
    4 Extracorporeal Assays of Thrombosis
    5 Platelet Life Span and Apoptosis
    6 A Live Cell Micro-imaging Technique to Examine Platelet Calcium Signaling Dynamics Under Blood Flow
    7 Platelet Shape Change and Spreading
    8 Clot Retraction
    9 Visualization and Manipulation of the Platelet and Megakaryocyte Cytoskeleton
    10 Measurement of Platelet Microparticles
    11 Assessing Protein Synthesis by Platelets 141
    12 A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies 155
    PART II ADDITIONAL PROTOCOLS FOR THE STUDY OF MEGAKARYOCYTE FUNCTION
    13 Characterization of Megakaryocyte Development in the Native
    Bone Marrow Environment 175
    14 Culture of Murine Megakaryocytes and Platelets from Fetal Liver and Bone Marrow
    15 In Vitro Generation of Megakaryocytes and Platelets from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells 205
    16 Megakaryocyte and Platelet Production from Human Cord Blood Stem Cells 219
    17 Culture of Megakaryocytes and Platelets from Subcutaneous Adipose Tissue and a Preadipocyte Cell Line 249
    18 Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
    19 Assessment of Megakaryocyte Migration and Chemotaxis 275
    PART III PERSPECTIVES ON THE STUDY OF PLATELET AND MEGAKARYOCYTE FUNCTION
    20 Megakaryopoiesis and Thrombopoiesis: An Update on Cytokines and Lineage Surface Markers
    21 Using Zebrafish (Danio rerio) to Assess Gene Function in Thrombus Formation
    22 Platelet Receptor Shedding
    23 Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
    24 Platelet Proteomics: State of the Art and Future Perspective 367
    Index 401