Kinase inhibitors : methods and protocols / edited by Bernhard Kuster. — New York : Humana Press : Springer, c2012. – (58.17/M592/v.795) |
Contents
Contents
Preface
Contributors.
1 Targeting Cancer with Small-Molecular-Weight Kinase Inhibitors
2 Small-Molecule Protein and Lipid Kinase Inhibitors in Inflammation and Specific Models for Their Evaluation
3 Measuring the Activity of Leucine-Rich Repeat Kinase 2: A Kinase Involved in Parkinson's Disease
4 Measuring PI3K Lipid Kinase Activity
5 A Fluorescence Polarization Assay for the Discovery of Inhibitors of the Polo-Box Domain of Polo-Like Kinase 1
6 Assessment of Hepatotoxicity Potential of Drug Candidate Molecules Including Kinase Inhibitors by Hepatocyte Imaging Assay Technology and Bile Flux Imaging Assay Technology 83
7 Kinase Inhibitor Selectivity Profiling Using Differential Scanning Fluorimetry
8 Chemoproteomic Characterization of Protein Kinase Inhibitors Using Immobilized ATP 119
9 Proteome-Wide Identification of Staurosporine-Binding Kinases Using Capture Compound Mass Spectrometry 135
10 Affinity Purification of Proteins Binding to Kinase Inhibitors Immobilized
on Self-Assembling Monolayers
11 Kinase Inhibitor Profiling Using Chemoproteomics 161
12 Covalent Cross-Linking of Kinases with Their Corresponding Peptide Substrates
13 Receptor Tyrosine Kinase Inhibitor Profiling Using Bead-Based Multiplex Sandwich Immunoassays 191
14 Monitoring Phosphoproteomic Response to Targeted Kinase Inhibitors Using Reverse-Phase Protein Microarrays.
15 Measuring Phosphorylation-Specific Changes in Response to Kinase Inhibitors in Mammalian Cells Using Quantitative Proteomics 217
16 Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays
Index 255