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Nanoproteomics : methods and protocols / edited by Steven A. Toms, Robert J. Weil. — New York : Humana Press, c2011. – (58.17/M592/v.790) |
Contents
Contents
PART I PRELIMINARY SAMPLE PREPARATION
1 MALDI-TOF Serum Profiling Using Semiautomated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads
2 Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling
PART II NANOSCALE SEPARATION
3 Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces
4 Three-Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures
5 Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species
6 Nanoelectrospray-MS of Native and Permethylated Glycans
7 N-Linked Global Glycan Profiling by NanoLC Mass Spectrometry
8 Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression， Protein-Protein Interaction， and Protein Phosphorylation
9 Nano LC-MS/MS: A Robust Setup for Proteomic Analysis
10 Nanofluidic Devices for Rapid Continuous-Flow Bioseparation
11 Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring
PART III NANOMATERIALS: NANOSTRUCTURED SURFACES
12 A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry 167
13 Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry
14 Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry
15 Protein Nanoarrays for High-Resolution Patterning of Bacteria on Gold Surfaces
PART IV NANOMATERIALS: NANOSCALE TOOLS FOR PROTEOMIC DISCOVERY
16 Engineered Multifunctional Nanotools for Biological Applications 203
17 Selective Capture of Phosphopeptides by Zirconium Phosphonate-Magnetic Nanoparticles
18 Nanowire Biosensors for Label-Free， Real-Time， Ultrasensitive Protein Detection 223
19 Generation ofAnti-infectome/Anti-proteonle Nanobodies 239
PART V NANOTECHNOLOGY" TECHNIQUES IN PROTEOMICS -- POTENTIAL APPLICATION FOR HEALTH AND DISEASE
20 Isolation， Propagation， and Analysis of Biological Nanoparticles
21 Functionalized Soluble Nanopolymers for Phosphoproteome Analysis
22 Elucidating Structural Dynamics of Integral Membrane Proteins on Native Cell Surface by Hydroxyl Radical Footprinting and Nano LC-MS/MS 287
23 Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography to the Characterization of Proteome， Glycoproteome， and Phosphoproteome Using Nano LC-MS/MS
Index 319
