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Approaches to the conformational analysis of biopharmaceuticals / Roger L. Lundblad. — Boca Raton : CRC Press, c2010. – (63.34/L962) |
Contents
Contents
Series Preface ix
Preface xi
Author xiii
Chapter 1 Introduction to Biopharmaceutical Conformational Analysis: Issues and Methods 1
References 5
Chapter 2 Comparability of Biotechnological/Biological Products and Biological Generics 19
References 29
Chapter 3 Application of Native Electrophoresis for the Study of Protein Conformation 37
References 41
Chapter 4 Affinity Chromatography Including Hydrophobic Interaction Chromatography in the Study of Biopolymer Conformation 49
References 64
Chapter 5 Size-Exclusion Chromatography and Biomolecular Conformation 77
References 83
Chapter 6 Use of Analytical Ultracentrifugation to Study Biomolecular Conformation
References 91
Chapter 7 Use of Differential Scanning Calorimetry to Measure Conformational Change in Proteins and Other Biomacromolecules 97
References 104
Chapter 8 Light Scattering and Biomacromolecular Conformation 111
References
Chapter 9 Use of luminescence to measure conformational change in biopharmaceuticals with emphasis on protein and protein drug products
References
Chapter 10 Near-Infrared Spectroscopy and Macromolecular Conformation
References
Chapter 11 Use of Mid-Infrared and Fourier Transform Infrared Spectroscopy to Study Conformation of Biomacromolecules 175
References 183
Chapter 12 Use of Raman Spectroscopy to Evaluate Biopharmaceutical Conformation
References
Chapter 13 Use of UV-VIS Spectrophotometry for the Characterization of Biopharmaceutical Products
References
Chapter 14 Use of optical rotatory dispersion and circular dichroism to study therapeutic biomacromolecule conformation
References
Chapter 15 Use of Nuclear Magnetic Resonance for the Characterization of Biotherapeutic Products
References
Chapter 16 Use of Chemical Probes for the Study of Protein Conformation
References
Chapter 17 Use of Hydrogen Exchange in the Study of Biopharmaceutical Conformation
References 245
Chapter 18 Use of Chemical Modification for the Conformational Analysis of Biopharmaceuticals 251
References 281
Chapter 19 Use of Immunology to Characterize Biopharmaceutical Conformation 299
References 307
Chapter 20 Use of Limited Proteolysis to Study the Conformation of Proteins of Biotechnological Interest 317
References 321
Chapter 21 Other Technologies for the Characterization of Conformational Change in Biopharmaceuticals 327
Crystallographic Analysis 327
Small-Angle Neutron Scattering and Small-Angle X-Ray Scattering 329
Equilibrium Dialysis 330
Enzyme Kinetics 330
References 333
Chapter 22 Development of an Experimental Approach for the Study of the Conformation of a Biological Therapeutic Product 341
References 344
Index 349
