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Methods in cell biology. v. 96, Electron microscopy of model systems / edited by Thomas Muller-Reichert. — Amsterdam : Elsevier, c2010. – (58.1574/M592/v.96)

Contents

    CONTENTS
    
    Preface
    Acknowledgments
    1. Electron Microscopy of Viruses
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    References
    2. Bacterial TEM: New Insights from Cryo-Microscopy
    I. Introduction
    II. Methods Involving Dehydration and Metal Stains
    III. Cryo-Electron Microscopy
    References
    3. Analysis of the Ultrastructure of Archaea by Electron Microscopy
    I. Introduction
    II. Growth of Cells for Electron Microscopy
    III. Preparation for Transmission Electron Microscopy
    IV. Preparation for Scanning Electron Microscopy
    V. Instrumentation and Methods
    VI. Conclusions
    References
    4. Chlamydomonas: Cryopreparation Methods for the 3-D Analysis of Cellular Organelles
    I. Introduction and Historical Perspectives
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Summary
    References
    5. Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    References
    6. Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    References
    7. Electron Microscopy of Paramecium (Ciliata)
    I. Introduction
    II. Methods
    III. Final Remarks
    References
    8. Ultrastructural Investigation Methods for Trypanosoma brucei
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Discussion
    VI. Summary
    References
    9. Dictyostelium discoideum: A Model System for Ultrastructural Analyses of Celt Motility and Development
    I. Introduction
    II. Current Approaches
    III. Future Challenges
    IV. Summary Comments
    References
    10. Toward Sub-second Correlative Light and Electron Microscopy of Saccharomyces cerevisiae
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results
    VI. Discussion
    References
    11. Fission Yeast: A Cellular Model Well Suited for Electron Microscopy Investigations
    I. Introduction
    II. Rationale
    III. EM Methods
    IV. Instrumentation, Materials, and Reagents
    V. Schizosaccharomyces pombe: Some Major Advances using EM
    VI. Discussion and Outlook
    VII. Summary
    References
    12. Electron Microscopy and High-Pressure Freezing of Arabidopsis
    I. Introduction
    II. Rationale
    II1. Methods
    IV. Materials
    V. Discussion
    VI. Concluding Remarks
    References
    13. Preparation Techniques for Transmission Electron Microscopy of Hydra
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results and Discussion
    VI. Concluding Remarks
    References
    14. Electron Microscopy of Flatworms: Standard and Cryo-Preparation Methods
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results and Discussion
    VI. Concluding Remarks
    References
    15. Three-Dimensional Reconstruction Methods for Caenorhabditis elegans Ultrastructure
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials and Instrumentation
    V. Discussion
    References
    16. Insects as Model Systems in Cell Biology
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results and Discussion
    VI. Concluding Remarks
    VII. Summary
    References
    17. Electron Microscopy of the Amphibian Model Systems Xenopus laevis and Ambystoma mexicanum
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Discussion and Outlook
    References
    18. Modern Approaches for Ultrastructural Analysis of the Zebrafish Embryo
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Concluding Remarks and Perspectives
    References
    19. Transmission Electron Microscopy of Cartilage and Bone
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Concluding Remarks
    References
    20. Electron Microscopy of the Mouse Central Nervous System
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results and Discussion
    VI. Concluding Remarks
    References
    21. Rapidly Excised and Cryofixed Rat Tissue
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results and Discussion
    References
    22. The Actin Cytoskeleton in Whole Mount Preparations and Sections
    I. Introduction and Rationale
    II. Whole Mount Techniques
    III. Sectioning Techniques
    IV. Summary
    References
    23. Three-Dimensional Cryo-Electron Microscopy on Intermediate Filaments
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Discussion
    References
    24. Correlative Time-Lapse Imaging and Electron Microscopy to Study Abscission in HeLa Cells
    I. Introduction
    II. Rationale
    III. Methods
    IV. Instrumentation and Materials
    V. Discussion
    References
    25. Viral Infection of Cells in Culture--Approaches for Electron Microscopy
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Discussion
    References
    26. Intracellular Membrane Traffic at High Resolution
    I. Abstract
    II. Introduction
    III. Methods
    IV. Outlook
    V. Materials and Equipment
    References
    27. 3D Versus 2D Cell Culture: Implications for Electron Microscopy
    I. Introduction
    II. Rationale
    III. Methods
    IV. Materials
    V. Results
    VI. Discussion
    VII. Synopsis
    References
    28. "Tips and Tricks" for High-Pressure Freezing of Model Systems
    I. Introduction
    II. Rationale
    III. Methods
    References
    Subject Index
    Volumes in Series