Methods in cell biology. v. 96, Electron microscopy of model systems / edited by Thomas Muller-Reichert. — Amsterdam : Elsevier, c2010. – (58.1574/M592/v.96) |
Contents
CONTENTS
Preface
Acknowledgments
1. Electron Microscopy of Viruses
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
2. Bacterial TEM: New Insights from Cryo-Microscopy
I. Introduction
II. Methods Involving Dehydration and Metal Stains
III. Cryo-Electron Microscopy
References
3. Analysis of the Ultrastructure of Archaea by Electron Microscopy
I. Introduction
II. Growth of Cells for Electron Microscopy
III. Preparation for Transmission Electron Microscopy
IV. Preparation for Scanning Electron Microscopy
V. Instrumentation and Methods
VI. Conclusions
References
4. Chlamydomonas: Cryopreparation Methods for the 3-D Analysis of Cellular Organelles
I. Introduction and Historical Perspectives
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Summary
References
5. Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum
I. Introduction
II. Rationale
III. Methods
IV. Materials
References
6. Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
7. Electron Microscopy of Paramecium (Ciliata)
I. Introduction
II. Methods
III. Final Remarks
References
8. Ultrastructural Investigation Methods for Trypanosoma brucei
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
VI. Summary
References
9. Dictyostelium discoideum: A Model System for Ultrastructural Analyses of Celt Motility and Development
I. Introduction
II. Current Approaches
III. Future Challenges
IV. Summary Comments
References
10. Toward Sub-second Correlative Light and Electron Microscopy of Saccharomyces cerevisiae
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results
VI. Discussion
References
11. Fission Yeast: A Cellular Model Well Suited for Electron Microscopy Investigations
I. Introduction
II. Rationale
III. EM Methods
IV. Instrumentation, Materials, and Reagents
V. Schizosaccharomyces pombe: Some Major Advances using EM
VI. Discussion and Outlook
VII. Summary
References
12. Electron Microscopy and High-Pressure Freezing of Arabidopsis
I. Introduction
II. Rationale
II1. Methods
IV. Materials
V. Discussion
VI. Concluding Remarks
References
13. Preparation Techniques for Transmission Electron Microscopy of Hydra
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results and Discussion
VI. Concluding Remarks
References
14. Electron Microscopy of Flatworms: Standard and Cryo-Preparation Methods
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results and Discussion
VI. Concluding Remarks
References
15. Three-Dimensional Reconstruction Methods for Caenorhabditis elegans Ultrastructure
I. Introduction
II. Rationale
III. Methods
IV. Materials and Instrumentation
V. Discussion
References
16. Insects as Model Systems in Cell Biology
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results and Discussion
VI. Concluding Remarks
VII. Summary
References
17. Electron Microscopy of the Amphibian Model Systems Xenopus laevis and Ambystoma mexicanum
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion and Outlook
References
18. Modern Approaches for Ultrastructural Analysis of the Zebrafish Embryo
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Concluding Remarks and Perspectives
References
19. Transmission Electron Microscopy of Cartilage and Bone
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Concluding Remarks
References
20. Electron Microscopy of the Mouse Central Nervous System
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results and Discussion
VI. Concluding Remarks
References
21. Rapidly Excised and Cryofixed Rat Tissue
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results and Discussion
References
22. The Actin Cytoskeleton in Whole Mount Preparations and Sections
I. Introduction and Rationale
II. Whole Mount Techniques
III. Sectioning Techniques
IV. Summary
References
23. Three-Dimensional Cryo-Electron Microscopy on Intermediate Filaments
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
References
24. Correlative Time-Lapse Imaging and Electron Microscopy to Study Abscission in HeLa Cells
I. Introduction
II. Rationale
III. Methods
IV. Instrumentation and Materials
V. Discussion
References
25. Viral Infection of Cells in Culture--Approaches for Electron Microscopy
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
References
26. Intracellular Membrane Traffic at High Resolution
I. Abstract
II. Introduction
III. Methods
IV. Outlook
V. Materials and Equipment
References
27. 3D Versus 2D Cell Culture: Implications for Electron Microscopy
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Results
VI. Discussion
VII. Synopsis
References
28. "Tips and Tricks" for High-Pressure Freezing of Model Systems
I. Introduction
II. Rationale
III. Methods
References
Subject Index
Volumes in Series