Molecular chaperones : methods and protocols / edited by Stuart K. Calderwood, Thomas L. Prince. — New York : Humana Press, c2011. – (58.17/M592/v.787)

Contents

    Contents
    
    Preface
    Contributors
    1 Targeted Deletion of Hsfl， 2， and 4 Genes in Mice
    2 The Role of Heat Shock Factors in Stress-Induced Transcription 21
    3 Hsp90 and Client Protein Maturation 33
    4 The Role of p23， Hop， Immunophilins， and Other Co-chaperones in Regulating Hsp90 Function 45
    5 Detecting HSP90 Phosphorylation
    6 Role of Molecular Chaperones in Biogenesis of the Protein Kinome 75
    7 Nucleotide Exchange Factors for Hsp70 Chaperones .
    8 Reconstitution of CHIP E3 Ubiquitin Ligase Activity 93
    9 Structure-Functions of HspB 1 (Hsp27)
    10 Combined Lentiviral and RNAi Technologies for the Delivery and Permanent Silencing of the hsp25 Gene 121
    11 Quantification of liSP27 and HSP70 Molecular Chaperone Activities 137
    12 Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
    13 Analysis of Heat-Shock Protein Localisation Using Flow Cytometry 155
    14 Quantitation of Heat-Shock Proteins in Clinical Samples Using Mass Spectrometry
    15 Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks 189
    16 Hsp70: Anti-apoptotic and Tumorigenic Protein 205
    17 Determination of Cell Survival or Death 231
    18 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer 245
    19 Preparation of a Heat-Shock Protein 70-Based Vaccine from DC-Tumor Fusion Cells
    20 Isolation of Heat Shock Protein Complexes 267
    21 Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat-Shock Protein 277
    22 Investigating Receptors for Extracellular Heat Shock Proteins
    23 Analysis of Cellular Migration Using a Two-Chamber Methodology 303
    Index 319