Laser capture microdissection : methods and protocols / edited by Graeme I. Murray. — 2nd ed. — New York : Humana Press, c2011. – (58.17/M592/v.755) |
Contents
CONTENTS
Preface
Contributors
1 Laser Capture Microdissection: Methods and Applications 1
2 Laser Microdissection for Gene Expression Profiling 17
3 Gene Expression Using the PALM System
4 Immunoguided Microdissection Techniques
5 Optimized RNA Extraction from Non-deparaffinized, Laser-Microdissected Material
6 Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue 77
7 MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue 85
8 Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh-Frozen Tissue Sections 95
9 Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue 107
10 Detection and Quantification of MicroRNAs in Laser-Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues 119
11 Laser Capture Microdissection Applications in Breast Cancer Proteomics 143
12 Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS 155
13 LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues 165
14 Purification of Diseased Cells from Barrett's Esophagus and Related Lesions by Laser Capture Microdissection 181
15 Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis
16 Application of Laser Microdissection and Quantitative PCR to Assess the Response of Esophageal Cancer to Neoadjuvant Chemo-Radiotherapy 197
17 Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer 203
18 Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE) 223
19 Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers 233
20 Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to Analyze EzH2 by Western Blot Analysis 245
21 Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR 257
22 Subcellular Renal Proximal Tubular Mitochondrial Toxicity with Tenofovir Treatment 267
23 Application of Laser-Capture Microdissection to Study Renal Carcinogenesis
24 Laser-Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer 291
25 Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies 301
26 Microdissection of Gonadal Tissues for Gene Expression Analyses
27 Duplex Real-Time PCR Assay for Quantifying Mitochondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells
28 Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection
29 Region-Specific In Situ Hybridization-Guided Laser-Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification 345
30 UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Postmortem Parkinson's Disease Brains 363
31 Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays 375
32 Probing the CNS Microvascular Endothefium by Immune-Guided Laser-Capture Microdissection Coupled to Quantitative RT-PCR 385
33 Laser-Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues 395
34 Laser-Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd-Chiari Syndrome 405
35 Laser-Capture Microdissection of Hyperlipidemic/ApoE-/- Mouse Aorta Atherosclerosis 417
36 Gene Expression Profiling in Laser-Microdissected Bone Marrow Megakaryocytes
37 Specific RNA Collection from the Rat Endolymphatic Sac by Laser-Capture Microdissection (LCM): LCM of a Very Small Organ Surrounded by Bony Tissues 441
38 The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis 449
39 Laser-Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues 461
40 Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia 477
Index 491