Chloroplast research in arabidopsis : methods and protocols. v. 1 / edited by R. Paul Jarvis. — New York : Humana Press, c2011. – (58.17/M592/v.774)

Contents

    Contents
    
    Preface
    Contributors
    PART I GENETICS， CYTOLOGY， AND IN VIVO ANALYSIS
    1 Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis， Following Chemical or Insertional Mutagenesis
    2 Analysis of Plastid Number， Size， and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy
    3 Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins
    4 Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts
    5 Visualisation of Stromules on Arabidopsis Plastids
    6 Analysis of Chloroplast Movement and Relocation in Arabidopsis
    7 Studying Starch Content and Sedimentation of Amyloplast Statoliths in Arabidopsis Roots
    8 Studying Arabidopsis Chloroplast Structural Organisation Using Transmission Electron Microscopy
    9 Transplastomics in Arabidopsis: Progress Toward Developing an Efficient Method
    PART II GENE EXPRESSION AND PROTEIN ACCUMULATION
    10 Isolation， Quantification， and Analysis of Chloroplast DNA 151
    11 Measurement of Transcription Rates in Arabidopsis Chloroplasts
    12 Studying the Structure and Processing of Chloroplast Transcripts
    13 In Vitro RNA-Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts
    14 Studying Translation in Arabidopsis Chloroplasts 209
    15 Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts 225
    PART III PROTEIN TRANSPORT， LOCALIZATION， AND TOPOLOGY
    16 In Silico Methods for Identifying Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins 243
    17 Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays 281
    18 Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions 307
    19 Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
    20 Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction 339
    21 Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases 351
    Index 369