Nitrogen fixation : methods and protocols / edited by Markus W. Ribbe. — New York : Humana Press, c2011. – (58.17/M592/v.766)

Contents

    Contents
    
    Preface
    Contributors
    SECTION I NITROGEN FIXATION
    1. Historic Overview of Nitrogenase Research
    2. Mechanism of Mo-Dependent Nitrogenase
    3. Assembly of Nitrogenase MoFe Protein 31
    4. Genomic Analysis of Nitrogen Fixation 49
    5. Enzymatic Systems with Homology to Nitrogenase 67
    SECTION II GENETIC AND BIOCHEMICAL METHODS
    6. Molecular Biology and Genetic Engineering in Nitrogen Fixation 81
    7. Purification of Nitrogenase Proteins
    8. Assays of Nitrogenase Reaction Products
    9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase
    SECTION III SPECTROSCOPIC METHODS
    10. X-Ray Crystallography
    11. X-Ray Absorption Spectroscopy 165
    12. Small Angle X-Ray Scattering Spectroscopy
    13. Electron Paramagnetic Resonance Spectroscopy
    14. Magnetic Circular Dichroism Spectroscopy
    15. Mossbauer Spectroscopy 221
    SECTION IV CHEMICAL METHODS
    16. Protocols for Cofactor Isolation of Nitrogenase 239
    17. Techniques for Functional and Structural Modeling of Nitrogenase 249
    SECTION V THEORETICAL CALCULATIONS
    18. Nitrogenase Structure and Function Relationships by Density Fnnctional Theory
    19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory
    Index 313