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新书资源(2012年3月)

Plant transcription factors : methods and protocols / edited by Ling Yuan, Sharyn E. Perry. — New York : Humana Press, c2011. – (58.17/M592/v.754)

Contents

    Contents
    
    SECTION I: INTRODUCTION
    1. MADS and More: Transcription Factors That Shape the Plant
    SECTION II: IDENTIFICATION OF TRANSCRIPTION FACTORS
    2. In Silico Mining and PCR-Based Approaches to Transcription Factor Discovery in Non-model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers
    3. Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System 45
    4. A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis
    SECTION III: FUNCTIONAL VERIFICATION
    5. CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors
    6. Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts
    7. Microarray-Based Identification of Transcription Factor Target Genes
    SECTION IV: PROTEIN-PROTEIN INTERACTION AND OTHER MEANS FOR ACTIVITY CONTROL
    8. Yeast Protein-Protein Interaction Assays and Screens
    9. Mapping Functional Domains of Transcription Factors 167
    10. Bimolecular Fluorescence Complementation as a Tool to Study Interactions of Regulatory Proteins in Plant Protoplasts 185
    11. Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification 195
    12. Assaying Transcription Factor Stability
    13. How to Assess the Intercellular Trafficking of Transcription Factors 235
    SECTION V: PROTEIN-DNA INTERACTION
    14. SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a Powerful Tool for Deciphering the Protein-DNA Interaction Space 249
    15. Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes
    16. Chromatin Immunoprecipitation to Verify or to Identify In Vivo Protein-DNA Interactions
    17. Visualizing and Characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors 293
    18. Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method 307
    SECTION VI: ENGINEERING METABOLIC PATHWAYS USING TRANSCRIPTION FACTORS
    19. Directed Evolution Through DNA Shuffling for the Improvement and Understanding of Genes and Promoters
    Index