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Methods in cell biology. v.102, Recent advances in cytometry. Part A : Instrumentation, methods / edited by Zbigniew Darzynkiewicz ... [et al]. — Amsterdam : Academic Press, c2011. – (58.1574/M592/v.102)

Contents

            CONTENTS
    
    Contributors
    Preface to fifth edition
    PART A -- Instrumentation, Methods
    1. Introduction A: Recent Advances in Cytometry Instrumentation, Probes, and Methods--Review
    I. Preface
    II. Image Cytometry
    III. New Instrumentations
    IV. New Probes, Components, and Methods
    V. New Strategies for Data Analysis
    VI. Perspective
    References
    Section I. Down-sizing cytometry to "micro" dimension
    2. Droplet Microfluidics for High-throughput Analysis of Cells and Particles
    I. Introduction
    II. Droplet Microfluidics
    III. Detection Techniques and Methodologies in Droplet Microfluidics
    IV. High-Throughput Cell and Particle Analysis in Droplet Microfluidics
    V. Perspectives
    VI. Conclusions
    References
    3. Parallel Imaging Microfluidic Cytometer
    I. Introduction
    II. Background
    III. Instrument Design
    IV. Operating Methods
    V. Results
    VI. Conclusions
    References
    4. Microfluidic Systems for Live Cell Imaging
    I. Introduction
    II. Physical Properties of Microfluidic Cell Culture
    III. Microfabrication Methods
    IV. Flow Control
    V. Design Aspects
    VI. Example Applications
    VII. Conclusion
    References
    5. Rise of the Micromachines: Microfluidics and the Future of Cytometry
    I. Introduction
    II. The Smaller the Better: Microfluidics and Enabling Prospects for Single Cytomics
    III. Microflow Cytometry (μFCM)
    IV. Microfluidic Cell Sorting 0.tFACS)
    V. Real-Time Cell Analysis: Living Cell Microarrays and a Real-Time Physiometry on a Chip
    VI. Conclusions
    References
    6. Label-Free Resistive-Pulse Cytometry
    I. Introduction
    II. Resistive-Pulse Sensing
    III. Coulter Counter on a Chip
    IV. Multiparametric RPS for Cell Cytometry
    V. Device Fabrication and Experimental Methods
    VI. Cell Size
    VII. Cell-Surface Marker Screening
    VIII. Applications
    IX. Conclusion
    References
    Section II. Imaging cytometry
    7. Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry
    I. Introduction
    II. Definition of Quantitative Imaging Cytometry (QIC) and Key Features Distinguishing Imaging Cytometry Platforms
    III. Technical and Analytical Features of iGeneration Laser Scanning Cytometry
    IV. Selected Application Areas of LSC
    V. Concluding Remarks
    References
    8. Analytical Capabilities of the ImageStream Cytometer
    I. Introduction
    II. Background
    III. Methods
    IV. Applications of ImageStream System
    V. Future Directions
    References
    9. Laser Scanning Cytometry: Capturing the Immune System In situ
    I. Introduction
    II. Background: Laser Scanning Cytometry Technology for Quantitatively Imaging and Analyzing Immune Responses In situ
    III. Rationale for LSC Analysis of Antigen-Specific T cell Responses In vitro and In vivo
    IV. Detailed Protocols for Tracking Antigen-specific T Cell Responses
    V. Acquisition and Analysis of Data Using WinCyte Software
    VI. Results: Analysis of the Role of pERK Signaling in Antigen-Specific Priming of T Cells
    VII. Application of LSC Technology to Analysis of the Immune System in Health and Disease
    VIII. Concluding Remarks and Future Directions
    References
    10. Image Cytometry Analysis of Circulating Tumor Cells
    I. Introduction
    II. Background and Technical Considerations
    III. Image Cytometry: Methods and Results
    IV. Conclusions and Future Directions
    References
    11. Preclinical Applications of Quantitative Imaging Cytometry to Support Drag Discovery
    I. Introduction
    II. Specific Examples: Example 1 - High-content Automated Tissue Analysis of ZDF Rat Pancreas
    III. Example 2 - Analysis of Biomarkers in Tissue Microarrays
    IV. Conclusions and Future Directions
    References list
    12. Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies
    I. Introduction
    II. Materials
    III. Staining and Cytometric Analyses of FNAs or Culture Cell Lines
    IV. Critical Aspects of the Procedure
    V. Results and Discussion
    VI. Biological Information and Future Directions
    References
    13. Automation of the Buccal Micronucleus Cytome Assay Using Laser Scanning Cytometry
    I. Introduction
    II. Rationale
    III. Methods
    IV. Summary
    References
    14. Laser Scanning Cytometry of Mitosis: State and Stage Analysis
    I. Introduction
    II. Background
    III. Methods
    IV. Discussion
    References
    Section III. Instrumentation, new probes and methods
    15. Lasers in Flow Cytometry
    I. Introduction
    II. Laser Characteristics for Flow Cytometry
    III. Laser Safety
    IV. Laser Diodes
    V. Diode-Pumped Solid State (DPSS) Lasers
    VI. Lasers by Wavelength
    VII. Multiwavelength Sources for Flow Cytometry
    VIII. Summary
    References
    16. The Use of Hollow Fiber Membranes Combined with Cytometry in Analysis of Bacteriological Samples
    I. Introduction
    II. Assessment of Membrane Suitability For Encapsulation of Microorganisms
    III. The Release of Bacteria Products
    IV. Production and Release by Bacteria of Biologically Active Factor(s)
    V. Conclusion
    References
    17. Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
    I. Introduction
    II. Major Characteristics of FPs
    III. Modem Advanced Red-Shifted FPs
    IV. Simultaneous Detection of Multiple FPs
    V. Fluorescent Timers
    VI. FRET-Based Genetically Encoded Biosensors
    VII. Biosensors Consisting of a Single FP
    VIII. Perspectives
    References
    18. Quantum Dot Technology in Flow Cytometry
    I. Introduction
    II. Fundamental Aspects of QD Flow Cytometry: Fluorescence and Hardware
    III. Utility of QDs in Multicolor Flow Cytometry
    IV. QD Conjugation to Antibodies
    V. Developing Staining Panels with QDs
    VI. Troubleshooting QD Use
    VII. Applications for QDs
    VIII. Conclusion
    References
    19. Background-free Cytometry Using Rare Earth Complex Bioprobes
    I. Introduction
    II. Instrumentation Development
    III. Bioprobes Development
    IV. Conclusion
    References
    20. Surface-Enhanced Raman Scattering (SERS) Cytometry
    I. Introduction
    II. Multiparameter Fluorescence Measurements
    III. Raman Scattering in Cytometry
    IV. Reagents and Instrumentation
    V. SERS Cytometry Applications
    VI. Summary and Prospects
    References
    21. Recent Advances in Flow Cytometric Cell Sorting
    I. Introduction
    II. Single-Cell Deposition and Index Sorting
    III. Positional Sorting
    IV. Reflective Plate Sorting
    V. Summary
    References
    Index