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Cell migration : developmental methods and protocols / edited by Claire M. Wells, Maddy Parsons. — 2nd ed. — New York : Humana Press, c2011. – (58.17/M592/v.769)

Contents

    Preface
    
    Contributors
    1 Cell Migration: An Overview
    2 Scratch-Wound Assay 25
    3 HGF-Induced DU145 Cell Scatter Assay 31
    4 Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
    5 Imaging Cells Within 3D Cell-Derived Matrix 53
    6 Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro
    7 Boyden Chamber 87
    8 Transwell Invasion Assays 97
    9 Imaging Podosome Dynamics and Matrix Degradation 111
    10 Endothelial Cell Migration Under Flow 137
    11 In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments
    12 Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
    13 Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy
    14 Live Cell Fluorescence Microscopy Techniques 205
    15 Measuring Invasion in an Organotypic Model 223
    16 Analysis of Cell Migration Using Caenorhabditis elegans as a Model System 233
    17 Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
    18 Measuring Inflammatory Cell Migration in the Zebrafish
    19 Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement 277
    20 Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
    21 Simple Experimental and Spontaneous Metastasis Assays in Mice
    22 Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo 331
    23 Measuring Angiogenesis in Mice
    24 Time-Lapse Imaging of Chick Cardiac Precursor Cells .
    25 Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
    26 Fluorescence Recovery After Photobleaching
    27 Measuring FRET Using Time-Resolved FLIM
    28 Cell Migration in Confinement: A Micro-Channel-Based Assay
    29 Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
    30 Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays 449
    Index 461