Human embryonic stem cells : the practical handbook / editors, Stephen Sullivan, Chad A. Cowan and Kevin Eggan. — Hoboken, N.J. : Wiley ; Chichester : John Wiley [distributor], c2007. – (58.155/H918)

Contents

    Contents
    
    Foreword
    Preface
    List of Contributors
    Section 1: Obtaining and culturing human embryonic stem cells
    1 Organization and good aseptic technique in the human embryonic stem cell laboratory
    2 Sourcing established human embryonic stem cell lines
    3 Culture of human embryos for stem cell derivation
    4 Derivation of human embryonic stem cell lines
    5 Standard culture of human embryonic stem cells
    6 Chemically-defined culture of human embryonic stem cells 81
    Section 2: Characterization of human embryonic stem cells
    7 Phenotypic analysis of human embryonic stem cells
    8 Genetic and epigenetic analysis of human embryonic stem cells 107
    Section 3: Manipulation of human embryonic stem cells
    9 In vivo differentiation of human embryonic stem cells
    10 In vitro differentiation of human embryonic stem cells
    11 Differentiation of human embryonic stem cells into extraembryonic cell types
    12 Directed differentiation of human embryonic stem cells into endoderm:
     (a) Early endoderm cells
     (b) Hepatic cells
     (c) Pancreatic cells
    13 Directed differentiation of human embryonic stem cells into mesoderm:
     (a) Cardiomyocytes
    (b) Endothelial cells
    (c) Osteogenic cells
    (d) Hematopoietic in vivo repopulating cells
    (e) Lymphocytes
    (f) Myeloid cells
    14 Directed differentiation of human embryonic stem cells into ectoderm:
    (a) Forebrain neurons
    (b) Dopaminergic neurons
    (c) Spinal motor neurons
    15 (a) Gene targeting in human embryonic stem cells: Knock out and knock in by homologous recombination
     (b) RNA interference in human embryonic stem cells
     (c) Generation of human gene reporters using bacterial artificial chromosome recombineering
    Afterword
    Index